008477 STOCK Tg(RARE-Hspa1b/lacZ)12Jrt/J These transgenic mice express beta-galactosidase ( lacZ ) gene under the control of the retinoic acid responsive element (RARE). Retinoic acid signaling is indicated by beta-galactosidase activity.

(D–F) The RARE-LacZ reporter mouse contains a RA response element under the control of a minimal promoter and is used to visualize RA signaling. LacZ staining of an E13.5 embryo (D) demonstrates the spatiotemporal activity of RA signaling throughout the head and trunk including the eye, neural tube, and limbs.

Jan 11, 2011 · RARE-lacZ contains multiple copies of the efficient RARE found upstream of the RARb gene, which we demonstrate here by ChIP to be much more efficient than the putative RARE upstream of Stra8.

Jul 30, 2019 · Here, we describe the use of a RA response element (RARE)-lacZ reporter cell-based method, which is simple and cost effective, to measure RA levels in small pieces of tissue from the embryo.

Evidence suggests vitamin A (VA) supplementation favors the non-amyloidogenic pathway through upregulation of α-secretase. Originally used to map embryonic retinoic acid (RA) signaling, RARE-LacZ mice possess multiple LacZ genes controlled by retinoic acid response elements (RAREs).

Sep 6, 2016 · Most established reporter systems, both transgenic mice and cell lines, make use of the highly potent RA response element (RARE) upstream of the RAR-beta gene to drive RA-inducible expression of reporter genes, such as beta-galactosidase or luciferase.

Mar 22, 2022 · Here, we found that active retinoic acid (RA) signaling in peripheral Muller glia is necessary for the abnormally long survival of these peripheral cones. RA depletion by conditional knockout of RA synthesis enzymes, or overexpression of an RA degradation enzyme, abrogated the extended survival of peripheral cones.

Feb 22, 2018 · To assay for changes in RA activity in vivo and in vitro upon Chd7 loss, we used transgenic mice with RARE-lacZ and cells with RARE-luciferase and varying dosage of Chd7. RARE-lacZ–transgenic mice express lacZ as early as …

Sep 6, 2016 · Here we describe the quantitative determination of relative RA levels generated in embryos and neurosphere cultures using the F9 RARE-LacZ reporter cell line.

The F9 RARE-LacZ cell line 14 is a powerful system that can be used for detection of RA produced by tissue explants 12,14,17,18. It is sensitive to RA concentrations as low as 100 fM, with no non-specific response detected in cells untreated with RA 12,14,18 .