Interestingly, sequence analysis of Rac1 shows that Rac1 T108 within the 106 PNTP 109 motif is likely an extracellular signal-regulated kinase (ERK) phosphorylation site and that Rac1 also has an ERK docking site, 183 KKRKRKCLLL 192 (D site), at the C terminus.

Jun 23, 2020 · ERK-mediated paxillin phosphorylation can promote FAK recruitment to focal adhesions and locally stimulates RAC1, which is essential for focal adhesion function.

Dec 24, 2013 · Expression of a constitutively active form of Rac1 in these breast cancer models blocked PI3Ki-induced down-regulation of ERK phosphorylation, apoptosis, and mitigated PI3K inhibitor sensitivity in vivo.

The function of cell surface ERK activation was probed using a targeted ERK monobody to demonstrate a role in membrane protrusion that may be mediated by a Rac1-dependent process. The analysis presented uses elegant optical probes and is interesting.

Jul 1, 2003 · We have shown that ERK-MAPK signaling acts to regulate Rho and Rac activity in colon carcinoma cells and thereby plays a major role in cell motility and invasion. ERK-dependent transcriptional activation of the fosL1 gene leads to its product, Fra-1, repressing Rho-GTP levels through inactivation of β1-integrin signaling. This depression of ...

We describe two signaling events downstream of ERK-MAP kinase contributing to cell motility in colon carcinoma cells. The Fos family member Fra-1 is expressed in an ERK-dependent manner. Silencing of Fra-1 expression with short interfering RNAs leads to losses of cell polarization, motility, and inv …

Nov 13, 2024 · In this study, we demonstrated that TNFAIP2 promotes TNBC angiogenesis by activating the Rac1-ERK-AP1-HIF1α signaling axis. Under hypoxia, TNFAIP2 activates Rac1 and ERK sequentially....

Inhibition of either Rac1 or Cdc42 signaling in adherent cells mimics the loss of anchorage and efficiently induces apoptosis in both immortalized and primary cells. In both cases cell death is dependent on the wild-type p53 tumor suppressor and is accompanied by activation of endogenous p53.

Mar 19, 2020 · Mechanistically, Rac1 activates aldolase A and ERK signaling which up-regulates glycolysis and especially the non-oxidative pentose phosphate pathway (PPP). This leads to increased...

We show that the ERK-MAPK pathway downstream of oncogenic Ras can regulate the activity of the small GTPases Rac and RhoA and thereby contribute to tumor cell motility and invasion. ERK-MAPK signaling to RhoA is via transcriptional upregulation of …