Rab7A was transiently associated to neosynthetic TfR-containing post–Golgi vesicles but dissociated before fusion with the plasma membrane. Together, our study reveals a role for Rab7 in the biosynthetic secretory pathway of the TfR, highlighting the diversity of the secretory vesicles’ nature.

May 1, 2022 · Based on these data, we have generated a model that RAB7 is dispensable for EGFR endocytic trafficking from the cell surface to the intraluminal vesicles of the LE/MVB but is required for fusion of the LE/MVB and the lysosome.

RAB7 regulates extracellular vesicle secretion in cisplatin resistant cells. (A) 2008 and C13 cervical cancer cells were immunostained with anti-RAB7 (green) and anti-CD63 antibodies (red) Scale bar: 10 μm. (B) Immunofluorescence intensity of CD63 was quantified by ImageJ software.

Jan 8, 2021 · Rab7A was transiently associated to neosynthetic TfR-containing post-Golgi vesicles but dissociated before fusion with the plasma membrane. Together, our study reveals a role for Rab7 in the biosynthetic secretory pathway of the TfR, highlighting the diversity of the secretory vesicles' nature.

May 1, 2009 · Ultrastructural analysis reveals that RAB7 is not required for formation of intraluminal vesicles of the LE/MVB, since RAB7-deficient cells have an increased number of enlarged LE/MVBs densely packed with intraluminal vesicles.

Oct 24, 2011 · We find that the major organelle in the endo-lysosomal pathway, both in terms of population and cargo transport, is positive for Rab7 and LAMP1. Dextran, a fluid phase cargo, shifts from localization within all three populations of vesicles at 30 minutes and 1 hour to primarily LAMP1- and Rab7/LAMP1-vesicles at longer times.

Oct 15, 2014 · Vesicular/membrane trafficking essentially regulates the compartmentalization and abundance of proteins within the cells and contributes in many signalling pathways. This membrane transport in eukaryotic cells is a complex process regulated by a large and diverse array of proteins.

Mar 18, 2025 · Rab proteins, a family of small GTPases, regulate this process, ensuring vesicles carrying cargo reach the right destination at the right time. Their ability to switch between active and inactive states allows them to coordinate complex trafficking events essential for …

Feb 11, 2023 · We show that lysosomal inhibition leads to increased secretion of mitochondria in large extracellular vesicles (EVs). The EVs are produced in multivesicular bodies, and their release is independent of autophagy.

Mar 1, 2011 · Rab7–HOPs interaction cascade is engaged in early-to-late endosomal maturation; Rab7–RILP/ORP1L cascade controls endosomal vesicles MT minus-end directed transport or ER contact depending on cellular levels of cholesterol; Rab7–FYCO1 cascade regulates endosomal vesicles MT plus-end directed movement.