Aug 16, 1999 · We demonstrate that the SCV recruits pre-existing lysosomal glycoproteins (Lgps) in a rab7-dependent manner, without directly interacting with lysosomes. We also show the transient accumulation, in the vicinity of the SCV, of novel rab7- and Lgp-containing vesicles containing very low amounts of cathepsin D.

We found that the initial centripetal displacement of the SCV is due to recruitment by Rab7 of Rab7-interacting lysosomal protein (RILP), an effector protein that can simultaneously associate with the dynein motor complex.

Aug 16, 1999 · We show that rab7 is present on SCVs during the biogenesis phase, which is characterized by the enrichment in some Lgps and that the kinetics of maturation of the SCV is retarded in cells overexpressing a dominant‐negative form of the rab7 GTPase.

Jan 11, 2019 · Detailed MS/MS assays, in vitro SUMOylation assays and structural docking analysis revealed SUMOylation of RAB7 (RAB7A) specifically at lysine 175. A SUMOylation-deficient RAB7 mutant (RAB7 K175R) displayed longer half-life, was beneficial to SCV dynamics and functionally deficient.

Jan 11, 2019 · Being a master regulator of endolysosomal trafficking, RAB7 activation and its context is crucial for the fate of the vesicle it belongs to, whether it is part of the late endosome or of the SCV. The precise location of RAB7 and its interacting partners determines these fates.

SCVs acquire the late endosome markers Rab7 and Rab9, but are not enriched for the characteristic late endosomal/lysosomal markers cathepsin D, lysobisphosphatidic acid (LBPA), and mannose-6-phosphate receptor (M6PR) (Méresse et al., 1999; Brumell et al., 2001b; Knodler and Steele-Mortimer, 2005).

SCV maturation is characterized by selective interactions with the endocytic system. Bacterial vacuoles transiently acquire early and recycling endosome markers such as early endosomal antigen 1 (EEA1), transferrin receptor (TfnR), Rab5 and Rab11.

Mar 1, 2025 · This inhibition prevents Rab7-dependent SCV from being transported to lysosomes. The PhoP/Q regulators further inhibit interactions between SCV and lysosomes or late endosomes, contributing to the stability of the intermediate vacuole (Garvis et al., 2001).

Apr 12, 2002 · Rab7, which is thought to be required for association of LAMP-1 with the SCV, still associated with SCV in wortmannin-treated cells. We have therefore concluded that a 3-phosphoinositide-dependent step exists following recruitment of Rab7 to the SCV.

Like endocytic vesicles, the Salmonella-containing vacuoles (SCVs) undergo a maturation process that involves sequential acquisition of Rab5 and Rab7 and displacement toward the...