Plasmid EGFP-Rab7A Q67L from Dr. Qing Zhong's lab contains the insert Rab7A and is published in Proc Natl Acad Sci U S A. 2010 Nov 9. 107 (45):19338-43. This plasmid is …

Jan 24, 2018 · RAB7 (Q67L) further resulted in a twofold increase in TOM20–LAMP1 mitochondria–lysosome FRET intensity compared to wild-type RAB7 (n = 200 cells per …

Two mutants of RAB7 are widely used to study the function of this GTPase: a dominant negative mutant (RAB7 T22N) characterized by impaired nucleotide exchange, thus locking the protein …

Feb 2, 2008 · To this purpose, we transfected cells to express GFP-tagged Rab7, Rab7 T22N, Rab7 Q67L, Rab7 L129F, Rab7 N161T, and Rab7 V162M, and then we starved the cells and …

Mar 3, 2010 · We found that the Rab7 compound mutants largely rescued the FRAP defect seen in the Q67L mutant alone, suggesting that the Rab7 disease-causing mutations increase GTP …

While Rab7 depletion significantly reduced the breakdown of LDs during starvation (Figs. 2a, b), this impairment could be corrected by the re-expression of the wild-type and constitutively …

Plasmid GFP-Rab7Q67L from Dr. Gia Voeltz's lab contains the insert Rab7a and is published in Dev Cell. 2021 Jan 11;56 (1):52-66.e7. doi: 10.1016/j.devcel.2020.12.014. This plasmid is …

Jun 6, 2009 · To determine whether Rab7-Q67L could reverse the growth factor-independent cell survival of cells with reduced PKCδ activity, we introduced GFP-Rab7-Q67L into the cell lines …

Sep 17, 2024 · Rab7, which is localized to late endosomes, is one of the most well-known GTPases. ORP1L is recruited by Rab7 to facilitate the fusion of late endosomes and …

Rab7 has been localized to late endosomes and shown to be important in the late endocytic pathway. In addition, it has been shown to have a fundamental role in the cellular vacuolation …