Plasmid DsRed-rab7 DN from Dr. Richard Pagano's lab contains the insert RAB7 (Homo sapiens) and is published in J Clin Invest. 2002 Jun . 109(12):1541-50. This plasmid is available through Addgene.

Jul 16, 2022 · In the present study, we show that Rab7 binding is more efficient with DN RNF167 than with WT RNF167, indicating that substrate ubiquitination likely inhibits RNF167-Rab7 interaction. This could explain why we measured an increased overlap between Rab7 and DN RNF167 in HeLa cells.

Because Rab7-DN caused both accumulation and impaired motility of Nsg2 along dendrites, we posited that Rab7 is required for transport of predegradative endosomes to degradative lysosomes in the soma.

In this study, we have expressed Enhanced Green Fluorescent Protein (EGFP)–tagged Rab7 wt as well as active and dominant-negative mutant proteins and analyzed their effect on the localization and other properties of late endocytic structures by confocal and electron microscopy.

Rab7 executes control over effectors throughout endosomal maturation. Schematic demonstrating endosomal maturation from early endosomes (EEs) to late endosomes (LEs), lysosomes (LYSs), and autophagosomes (APs), each with respective Rab7 levels and domains (red domains).

Jun 1, 2022 · Expression of dominant-negative Rab7 (Rab7-DN) led to accumulation of NSG1/2 in somatic and dendritic endosomes because of reduced vesicle motility and failure to degrade NSG1/2 (Yap et al., 2018).

Rab7 is a late endosome-/lysosome-associated small GTPase, perhaps the only lysosomal Rab protein identified to date. Rab7 plays critical roles in the endocytic processes.

Oct 13, 2017 · In this study, we have expressed Enhanced Green Fluorescent Protein (EGFP)–tagged Rab7 wt as well as active and dominant-negative mutant proteins and analyzed their effect on the localization and other properties of late endocytic structures by confocal and electron microscopy.

Here we selected artificial proteins that modulate cellular proteins required for HPV infection and discovered that entry requires TBC1D5, a retromer-associated, Rab7-specific GTPase-activating protein.

Jun 6, 2009 · The Rab7 GTPase promotes membrane fusion reactions between late endosomes and lysosomes. In previous studies, we demonstrated that Rab7 inactivation blocks growth factor withdrawal-induced cell death. These results led us to …