Jan 17, 2018 · Retromer is an endosomal multi-protein complex that organizes the endocytic recycling of a vast range of integral membrane proteins. Here, we establish an additional retromer function in controlling the activity and localization of the late endosomal small GTPase RAB7.

In order to modulate RAB7 activity and prevent lysosomal accumulation, retromer should co‐localize with RAB7 near lysosomal membranes. Thus, we next tested how retromer and RAB7 spatially relate to each other and relative to lysosomes in wild‐type HeLa cells.

The age-related retardation of female fertility was improved after in vivo treatment of ML098. Thus, RAB7 activity is required to maintain the balance between mitophagy and chromosome stability and RAB7 activator is a good candidate to ameliorate age-related deterioration of …

Rab7 (or Ypt7 in yeast) is one of the well-characterized members of the Rab family small GTPases, which serve as master regulators of membrane trafficking in eukaryotes. It localizes to late endosomes and lysosomes and has multiple functions in the autophagic pathway as well as in the endocytic pathway.

Mechanistic studies indicate that NRBF2 is required for autophagosome trafficking toward the lysosome via regulating RAB7 activity by interacting with GEF CCZ1-MON1A and facilitating GEF activity. Interestingly, this mechanism is associated with APP-CTFs degradation and Aβ …

Knocking down TBC1D5 increased the expression of active Rab7, promoted the fusion of lysosome and autophagosome, thus improving mitophagy, and improved the morphology of hippocampal neurons and the impaired behavior in AD mice.

Nov 20, 2017 · RAB7 is known to be a key organizer/regulator of the late endosomal/lysosomal network that associates with various effectors to orchestrate diverse func-tions such as lysosomal biogenesis, late endosome–lysosome fusion, autophagosome maturation, and cargo transport within the late endocytic network (Guerra & Bucci, 2016).

Jun 25, 2018 · The authors show that loss of TBC1D5 leads to increased levels of GTP-bound Rab7a, increasing the endosomal localisation of the retromer CSC and enhancing its interaction with accessory proteins, such as Fam21 and other components of the WASH complex.

Nov 20, 2017 · Retromer is an endosomal multi‐protein complex that organizes the endocytic recycling of a vast range of integral membrane proteins. Here, we establish an additional retromer function in controlling the activity and localization of the late endosomal small GTP ase RAB 7.

Jun 6, 2009 · To determine whether Rab7-Q67L could reverse the growth factor-independent cell survival of cells with reduced PKCδ activity, we introduced GFP-Rab7-Q67L into the cell lines expressing dominant-negative PKCδ and PKCδ RNAi.