Feb 12, 2020 · By generating myogenic Rab5a-deficient mice, we uncover the essential roles of Rab5a in regulating skeletal muscle regeneration. We further reveal that Rab5a promotes myoblast differentiation and...

Apr 14, 2020 · We demonstrate efficient release of ligand-conjugated siRNA from vesicles damaged by small molecules, enhancing target knockdown up to ∼ 47-fold in tumor cells. Still, mismatch between...

May 13, 2008 · Depletion of the Rab5 guanine nucleotide exchange factor (GEF) Gapex-5 with short interfering RNA (siRNA) inhibited Rab5 activation during phagocytosis, whereas depletion of other Rab5 GEFs (Rabex-5 and Rin) did not.

Oct 30, 2009 · To test the possibility that the differential delay of EGFR trafficking caused by individual Rab5 isoform silencing corresponds to the relative isoform abundance, we examined the concentration of Rab5 isoforms in HeLa and DU145 cells using purified recombinant Rab5 isoform proteins as standards.

We generated Rab5 knockdown MDA-MB-231 cells using small interfering RNA (siRNA), and migration, invasion and exosome secretion were then determined to investigate the role of Rab5 in TNBC tumorigenesis and progression.

Small interfering RNA (siRNA)-mediated depletion of Rab5a expression attenuated the disruption of LPS-induced internalization of VE-cadherin and the disorder of cell polarity. Furthermore, knockdown of Rab5 inhibited the vascular endothelial hyperpermeability and protected endothelial barrier function from LPS injury, both in vitro and in vivo.

Jul 17, 2019 · To identify key regulators of HBV production, we performed short interfering RNA (siRNA) screening for Rab proteins, which are considered to act as molecular switches in vesicular trafficking using HepG2.2.15 cells. Among 62 Rab proteins, the suppression of Rab5B most significantly increased HBV DNA in the culture supernatant.

Rab5 is the master regulatory GTPase on early endosomes. Rab5 functions in endocytosis, endosome fusion, maturation, trafficking and signaling are dependent on its membrane localization and cycling between an active, GTP-bound and an inactive, GDP-bound state .

Apr 1, 2019 · Here we show that RAB5/7, which are involved in the endolysosomal pathway, play key roles in the maintenance of CSC survival via regulation of the mitophagic pathway. Inhibition of RAB5/7 efficiently eliminated colorectal CSCs and disrupted cancer foci.

To test the possibility that the differential delay of EGFR trafficking caused by individual Rab5 isoform silencing corresponds to the relative isoform abundance, we examined the concentration of Rab5 isoforms in HeLa and DU145 cells using purified recombinant Rab5 isoform proteins as …