Jan 1, 2005 · Here, we describe how to monitor the ability of GDI to extract Rab5 from early endosomal membranes in vitro and the role of p38 MAPK in this process. In addition, we detail …

Jan 1, 2001 · We have identified Rab5 as a critical cytosolic component required for clathrin-coated pit function. Given the well-established role of Rab5 in the fusion of endocytic vesicles …

Sep 19, 2004 · We present here the crystal structure of the Rab5 GTPase domain in complex with a GTP analog and the C-terminal domain of effector Rabaptin5.

Sep 19, 1997 · The small GTPase Rab5 plays an essential role in endocytic traffic. Rab GDP dissociation inhibitor delivers Rab5 to the membrane, where a nucleotide exchange activity …

in permeabilised cells, the Rab5–GDI complex is required for ligand sequestration into clathrin-coated pits. Conclusions: We have identified Rab5 as a critical cytosolic component required …

Jun 1, 2005 · Rab5-GDP in a complex with GDI promotes recruitment of transmembrane proteins into coated pits. The newly discovered GEF RME-6 is also localized to the plasma membrane …

Here, we describe how to monitor the ability of GDI to extract Rab5 from early endosomal membranes in vitro and the role of p38 MAPK in this process. In addition, we detail how to …

Jan 1, 1998 · We have purified this component and shown it to consist of a complex of Rab5 and GDI (Rab5–GDI). These results indicate a novel role for the Rab5–GDI complex at the very …

A detailed new structural and dynamic model for human Rab5(GDP) recognition and binding with human GDI at the early endosome membrane and in its dissociated state is presented. In the …

Feb 11, 2020 · The GTP-Rab is located on the plasma membrane, and GDP-Rab is located in the cytoplasm. The transformation between the activated and inactivated forms requires three …