Apr 19, 2010 · We find that TBC1D10A-C regulate exosome secretion in a catalytic activity-dependent manner. We show that Rab35 is the target of TBC1D10A-C and that the inhibition of Rab35 function leads to intracellular accumulation of …

Apr 4, 2010 · We find that TBC1D10A–C regulate exosome secretion in a catalytic activity–dependent manner. We show that Rab35 is the target of TBC1D10A–C and that the inhibition of Rab35 function leads to intracellular accumulation of endosomal vesicles and impairs exosome secretion.

Accordingly, inhibition of exosome secretion by the expression of the GAP was abolished when a GTP-blocked mutant of Rab35 was coexpressed with TBC1D10B. Conversely, expression of a dominant-negative Rab35 mutant or knockdown of Rab35 resulted in a decrease of exosome-associated PLP.

Jun 12, 2022 · This study demonstrated that the mitochondrial protein TRX2 interacted with Rab35 and induced Rab35 ubiquitination and degradation, resulting in impaired exosome secretion. These data revealed a new role for TRX2 in the regulation of exosome secretion and the upstream regulatory mechanism of Rab35.

Apr 19, 2010 · The GTPase Rab35 regulates the release of small vesicles called exosomes from the surface of glial cells, say Hsu et al. Exosomes are formed in the lumen of specialized endosomes called multivesicular bodies (MVBs), which fuse with the plasma membrane to secrete the vesicles extracellularly.

Apr 3, 2019 · HOTAIR promoted the release of exosomes by inducing MVB transport to the plasma membrane. HOTAIR regulated RAB35 expression and localization, which controlled the docking process. Moreover, HOTAIR facilitated the final step of fusion by influencing VAMP3 and SNAP23 colocalization.

Jun 12, 2022 · These results revealed a new role for TRX2 in the regulation of exosome secretion and cell migration and explained the upstream regulatory mechanism of Rab35. Furthermore, these findings also provide new molecular evidence …

NSCLC cell-derived exosomes with RAB35 knockdown or miR-185-5p overexpression (Enzyme treated) inhibited NSCLC cell migration and invasion. The exosomes secreted by A549 cells and H2170 cells were treated with enzyme and then added into the two kinds of cell models with downregulation of RAB35.

We show that Rab35 is the target of TBC1D10A–C and that the inhibition of Rab35 function leads to intracellular accumulation of endosomal vesicles and impairs exosome secretion. Rab35 localizes to the surface of oligodendroglia in a GTP-dependent manner, where it increases the density of vesicles, sug-gesting a function in docking or tethering.

Jun 21, 2016 · Since then, Rab35 has become one of the most studied Rabs involved in a growing number of cellular functions, including endosomal trafficking, exosome release, phagocytosis, cell migration, immunological synapse formation and neurite outgrowth.