RPMI 1640, simply known as RPMI medium, is a cell culture medium commonly used to culture mammalian cells. [1] RPMI 1640 was developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at Roswell Park Comprehensive Cancer Center (formerly known as Roswell Park Memorial Institute), from where it derives its name. [2]

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B …

Choose RPMI 1640 Media in standard, innovative, or custom formulations to fit your cell culture needs. RPMI 1640 is vitamin-rich and contains the reducing agent glutathione.

RPMI 1640 Medium is the only media that contains the reducing agent glutathione and high concentrations of vitamins and choline. It also contains vitamin B12, biotin, and PABA, which are not found in DMEM.

RPMI-1640 medium is commonly used for suspension cultures of mammalian and primary cells, such as HeLa cells, human peripheral blood mononuclear cells (PBMCs), human normal and neoplastic leukocytes, Jukrat cells, and more.

Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Roswell Park Memorial Institute (RPMI) medium or RPMI 1640 is used in cell culture, tissue culture, and also to improve the cellularity and morphology of CSF cytology specimens. To determine whether RPMI medium can be used to preserve cell morphology in pleural/peritoneal effusion samples.

Roswell Park Memorial Institute Medium, commonly referred to as RPMI medium, is a nutritive liquid used to support cell viability in biological samples. RPMI has no protein, so it is supplemented separately with bovine serum. It is not a fixative. Formalin, Cytolyt, and Zeus IF, for example, are fixatives.

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or …

RPMI-1640 was developed by Moore, et al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins.