RNA Interference in the Age of CRISPR: Will CRISPR Interfere with RNAi …
Tools of both CRISPR (gRNA/Cas9 carrying plasmids or gRNA as synthetic oligonucleotide along with Cas9 separately through plasmids) and RNAi (siRNA, or shRNA as plasmid or virus) can be introduced into nearly all mammalian cells in vitro, by optimizing the …
High-throughput functional genomics using CRISPR–Cas9
Apr 9, 2015 · In this Review, we describe recent advances using Cas9 for genome-scale screens, including knockout approaches that inactivate genomic loci and strategies that modulate transcriptional activity....
RNAi-mediated control of CRISPR functions - PMC
CRISPR-Cas9 has become a versatile tool for genome editing and regulation, and strategies to effectively control its activity have attracted much attention. RNAi, also a gene-regulating tool, is used as another mechanism by which eukaryotes resist the invasion of foreign genetic material.
Systematic comparison of CRISPR/Cas9 and RNAi screens for
May 9, 2016 · We compared the ability of short hairpin RNA (shRNA) and CRISPR/Cas9 screens to identify essential genes in the human chronic myelogenous leukemia cell line K562.
Highly efficient RNAi and Cas9-based auto-cloning systems for
Jun 19, 2018 · Both new vectors include a novel CRISPR/Cas9-based auto-cloning vector system rendering needless the use of restriction and ligase enzymes in generating DNA constructs. This novel, efficient RNAi and auto-cloning Cas9 systems …
Systematic comparison of CRISPR/Cas9 and RNAi screens for …
We compared the ability of short hairpin RNA (shRNA) and CRISPR/Cas9 screens to identify essential genes in the human chronic myelogenous leukemia cell line K562. We found that the precision of the two libraries in detecting essential genes was similar and that combining data from both screens impro …
RNA and CRISPR Interferences: Past, Present, and Future ... - PubMed
Such advances in RNA delivery and chemical modifications have benefited researchers who are developing gene-editing therapies based on CRISPR-Cas9, an RNA-guided endonuclease, which is already having a major impact on biology and medicine.
RNAi/CRISPR Screens: from a Pool to a Valid Hit - Cell Press
Aug 31, 2018 · CRISPR-associated protein 9 (Cas9) an RNA-guided DNA endonuclease that cleaves DNA to generate DSBs in a targeted manner, applied in combination with sgRNA for genome editing.
RNA-dependent RNA targeting by CRISPR-Cas9 - PMC - National …
Jan 5, 2018 · RNA-guided RNA cleavage is programmable and site-specific, and we find that this activity can be exploited to reduce infection by single-stranded RNA phage in vivo. We also demonstrate that Cas9 can direct PAM-independent repression of gene expression in bacteria.
Advances in CRISPR-Cas9 genome engineering: lessons learned from RNA ...
Mar 23, 2015 · We examine how the history of RNAi can inform today's challenges in CRISPR-Cas9 genome engineering such as efficiency, specificity, high-throughput screening and delivery for in vivo and therapeutic applications.