pSC101 is a DNA plasmid that is used as a cloning vector in genetic cloning experiments. pSC101 was the first cloning vector, used in 1973 by Herbert Boyer and Stanley Norman Cohen. Using this plasmid they have demonstrated that a gene from a frog could be transferred into bacterial cells and then expressed by the bacterial cells.

Low copy number plasmid from Salmonella. pSC101 was the first plasmid vector used for cloning. Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets. Sticky ends from different BsrDI sites may not be compatible. Efficient cleavage requires at least two copies of the RsrII recognition sequence.

Let’s consider pSC101. This plasmid is stringently controlled and has a low copy number. Like the E. coli chromosome, it has a single ori and requires the host proteins DnaA and IHF to initiate replication. But pSC101 requires a third component: RepA, a protein encoded by pSC101 itself.

The minimal replication origin (ori) of the plasmid pSC101 was defined as an about 220-bp region under the condition that the Rep (or RepA) protein, a plasmid-encoded initiator protein, was supplied in trans.

Plasmid pSC101-AmpR EV from Dr. Tanel Tenson's lab is published in Sci Rep. 2024 Mar 22;14(1):6883. doi: 10.1038/s41598-024-57597-8. This plasmid is available through Addgene.

Plasmid pSC101-Donor from Dr. Sheng Yang's lab contains the insert LE, RE, KanR, temperature sensitive pSC101 origin of replication and is published in ACS Synth Biol. 2020 Jun 17. doi: 10.1021/acssynbio.0c00073. This plasmid is available through Addgene.

Jan 25, 2018 · pSC101 is a narrow host range, low-copy plasmid commonly used for genetically manipulating Escherichia coli. As a byproduct of a genetic screen for a more sensitive lactam biosensor, we...

The origin of replication of plasmid pSC101 presents features reminiscent of those found in a number of plasmids. As for those plasmids, many details about the way it initiates its replication are beginning to be known, but the regulation of this process will not be easily understood.

The nucleotide sequence of a 770-bp ori region of plasmid pSC101 is presented. The sequence shows homologies to some parts of Escherichia coli oriC and phage G4 ori.

Provide pSC101 vector/plasmid map, full length sequence, antibiotic resistance, size and other information