This tool strain may be useful for generating tissue specific-targeted mutants for studying limb bud development and patterning.

Dec 1, 2022 · By conducting genetic lineage tracing and cell depletion experiments in newly generated knock-in Cre/CreERT2 lines, we show here that rare Prrx1 -expressing cells act as stem cells for bone,...

Paired-related homeobox 1 (Prx1) is expressed during skeletogenesis. We hypothesize that fracture recapitulates Prx1’s expression, and Prx1 expressing cells are critical to induce repair.

029211 B6.Cg-Tg (Prrx1-cre/ERT2,-EGFP)1Smkm/J This Prx1CreER-GFP mutant mouse strain carries a transgene with tamoxifen inducible Cre recombinase and may be useful for generating osteochondro progenitor cell specific targeted mutants for studies of cartilage and bone development, lineage fate mapping, and isolation of transgene expressing perios...

Dec 3, 2015 · Prior studies have shown that Prx1-Cre, Osx-Cre, Lepr-Cre, and Nes- Cre broadly target mesenchymal stromal cells in the bone marrow. Here, we rigorously define the stromal cell populations targeted by two Cre-transgenes that are commonly used to target osteolineage cells (Ocn-Cre, and Dmp1-Cre) and introduce a new Cre-transgene (Tagln-Cre) that ...

We have used a Prx1 limb enhancer to drive expression of Cre Recombinase in transgenic mice. This regulatory element leads to Cre expression throughout the early limb bud mesenchyme and in a subset of craniofacial mesenchyme.

Feb 19, 2024 · PRX1 (also known as PRRX1), paired-related homeobox-1, stands as an iconic marker of mesenchymal stem cells. 13 Extensive research has demonstrated the pivotal role played by PRX1-positive...

Apr 23, 2024 · We then crossed Prx1-cre mice with Mettl3 fl mice to generate Prx1-cre; Mettl3 fl/fl mice (Figure 2A). Mettl3 was efficiently deleted in limb bone marrow MSCs from two-week-old Prx1-cre; Mettl3 fl/fl mice, leading to a significant reduction in the …

Oct 10, 2007 · In this study, we address the function of Transforming Growth Factor beta (TGF-β) and its Type II receptor (Tgfbr2) in limb development in vivo. Mouse embryos were generated in which the Tgfbr2 gene was deleted in early limb mesenchyme using …

Apr 4, 2015 · Here, we show that the majority of the precursor and mature subcutaneous white adipocytes in adult C57Bl/6 mice are labeled by Prx1-Cre. In sharp contrast, few to no brown adipocytes or visceral white adipocytes are marked by Prx1-Cre.