Apr 5, 2018 · Here, we show that S. pombe rnh1∆ rnh201∆ cells, which lack the RNase H enzymes, accumulate R‐loops and activate DNA damage checkpoints. Their viability requires critical DSB repair proteins and Mus81, which resolves DNA junctions formed during repair of broken replication forks.

Here, we show that S. pombe rnh1∆ rnh201∆ cells, which lack the RNase H enzymes, accumulate R-loops and activate DNA damage checkpoints. Their viability requires critical DSB repair proteins and Mus81, which resolves DNA junctions formed during repair of broken replication forks.

When the two RNase H genes (rnh1 + and rnh201 +) in S. pombe were deleted in elf1Δ P cells, the frequency of suppressor generation in rich medium increased greater than fourfold from 0.18 to 0.85% (Figure 7A).

RNH1 (YMR234W) Saccharomyces cerevisiae References / Literature PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Schizosaccharomyces , we showed that RNA-DNA hybrids form as pombe part of the homologous-recombination (HR)-medi-ated DSB repair process and that RNase H enzymes are essential for their degradation and efficient completion of DNA repair.

Feb 10, 2021 · Here, we use polymerase usage sequencing to visualize in vivo replication dynamics of HR-restarted forks at an S. pombe replication barrier, RTS1, and model replication by Monte Carlo simulation.

Here, we show that S. pombe rnh1∆ rnh201∆ cells, which lack the RNase H enzymes, accumulate R-loops and activate DNA damage checkpoints. Their viability requires critical DSB repair proteins and Mus81, which resolves DNA junctions formed during repair of broken replication forks.

Here, we show that S. pombe rnh1∆ rnh201∆ cells, which lack the RNase H enzymes, accumulate R‐loops and activate DNA damage checkpoints. Their viability requires critical DSB repair proteins and Mus81, which resolves DNA junctions formed during repair of broken replication forks.

Jun 16, 2022 · Culture of Schizosaccharomyces pombe: For each experiment, aim for 10 7 cells/mL in 100 mL (i.e., 10 9 cells total). We recommend to systematically grow both rnh1 + rnh201+ and rnh1Δrnh201Δ cells as positive control. rnh1 + rnh201+ has a generation time of ~2.4 h in YES+A, while rnh1Δrnh201Δ has a generation time of ~3 h.

They demonstrate that S. pombe needs to both generate and destroy RNA:DNA hybrids in order to efficiently complete homologous recombination (HR). Too little RNase H, and the hybrids persist and inhibit completion of HR.