A polyhistidine-tag, best known by the trademarked name His-tag, is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C …

The polyhistidine affinity tag, also known as the His-tag or His 6, usually consists of six consecutive histidine residues, but can vary in length from two to ten histidine residues. …

Polyhistidine affinity tags are commonly placed on either the N or the C terminus of recombinant proteins. Optimal placement of the tag is protein specific. A potential problem is inaccessibility …

The polyhistidine tag (His-tag) is one of the most popular protein tags used in the life sciences. Traditionally, the detection of His-tagged proteins relies on immunoblotting with anti-His …

A Polyhistidine Tag is a short chain of six histidine residues that can be added precisely at either the C or N terminus of a protein molecule. This tag allows for the binding of the protein to a …

The polyhistidine- or His6-tag is a protein tag originally developed for efficient protein purification in 1988 (Hochuli, Bannwarth & Döbeli et al., 1988). As a result of its comparably great cost …

A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, …

The polyhistidine-tag (His-tag) is among the most prominent examples of affinity tags used for the isolation of recombinant proteins from their expression hosts. Short peptide tags are …

It is true that the most commonly used polyHis tag consists of 6 constitutive histidine residues. Longer or shorter polyHis tags have been used much infrequently, but they have been proven …

IMAC is a widely-used method for rapidly purifying polyhistidine affinity-tagged proteins, resulting in 100-fold enrichments in a single purification step. The chelators most commonly used as …