Replication slippage of the thermophilic DNA polymerases B and …
Here, we show that PolB and PolD replicative DNA polymerases from the archaeal model Pyrococcus abyssi (Pab) slip in vitro during replication of a single-stranded DNA template …
The Hyperthermophilic Euryarchaeota - ScienceDirect
Jul 1, 2005 · Here, we focus on the two identified DNA polymerases, the family B DNA polymerase B (Pab polB) and the family D DNA polymerase D (PabpolD) from the …
(PDF) Replication slippage of the thermophilic DNA
Aug 7, 2014 · Here, we show that PolB and PolD replicative DNA polymerases from the archaeal model Pyrococcus abyssi (Pab) slip in vitro during replication of a single-stranded DNA …
PCR performance of a thermostable heterodimeric archaeal DNA …
May 7, 2014 · Here we looked at the PCR performances of the proof-reading D-type DNA polymerase from P. abyssi, Pab-polD. Fragments, 3 kilobases in length, were specifically PCR …
Characterization of Family D DNA polymerase from
May 3, 2014 · All archaeal genomes except Crenarchaea, encode for a member of Family B (polB) and Family D (polD) DNA polymerases. Gene deletion studies in Thermococcus …
Processing of matched and mismatched rNMPs in DNA by …
Using Archaeal RER enzymes, RNase HII, DNA Pols (PolD and PolB), Lig1, and Fen1 in test substrate in vitro, we show that mispaired rNMPs can be repaired by the conserved RER. We …
rearrangements such as microsatellite instability. Here, we show that PolB and PolD replicative DNA polymerases from the archaeal model Pyrococcus abyssi (Pab) slip in vitro during …
Diversity and evolution of B-family DNA polymerases - PMC
B-family DNA polymerases (PolBs) represent the most common replicases. PolB enzymes that require RNA (or DNA) primed templates for DNA synthesis are found in all domains of life and …
Molecular Recognition of Canonical and Deaminated Bases
Aug 15, 2012 · Using Pyrococcus abyssi DNA B family polymerase (PabPolB), we investigated the discrimination between deaminated and natural nucleotide (s) by primer extension assays, …
The Hyperthermophilic Euryarchaeota Pyrococcus abyssi Likely …
Jul 1, 2005 · Here, we focus on the two identified DNA polymerases, the family B DNA polymerase B (Pab polB) and the family D DNA polymerase D (Pab polD) from the …