Plasmid pMDLg/pRRE from Dr. Didier Trono's lab contains the insert HIV-1 GAG/POL and is published in J Virol. 1998 Nov . 72(11):8463-71. This plasmid is available through Addgene.

Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence. SnapGene File: Plasmid sequence and SnapGene enhanced annotations. Use with SnapGene software or the free Viewer to visualize additional data and align other sequences.

3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)

Image: Illustrated plasmid map in PNG format GenBank File: Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence. SnapGene File: Plasmid sequence and SnapGene enhanced annotations. Use with SnapGene software or the free Viewer to visualize additional data and align other sequences.

pMDLg/pRRE 1kb Ladder Uncut This gel depicts the NEB 1kb ladder and a digestion of the plasmid pMDLg/pRRE with the following enzymes: single digests with BamHI and ClaI BamHI ClaI 0 .5 1 1.5 2 3 4 DNA digested with singel digests with BamHI and ClaI for 1 hour Gel: 0.8% TAE stained with 100 ml 1X TAE and 5uL of ethidium bromide

pMDLg/pRRE - This is a 3rd generation lentiviral packaging plasmid from the Trono lab that contains the gag and pol genes. It also requires pRSV-Rev and the envelope expressing plasmid pMD2.G . Find pMDLg/pRPE .

pMDLg/pRRE 3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259) 12253

Jun 5, 2015 · pMDLg/pRRE Citations (764) Originally described in: A third-generation lentivirus vector with a conditional packaging system. Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L J Virol. 1998 Nov . 72(11):8463-71.

3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)

Part of the 3rd generation packaging system. Please note that most of Trono lab lentiviral vectors can only be used with the 2nd generation packaging system. See notes for pMDLg/pRRE. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.