Jan 10, 2023 · Running undigested DNA on a gel as a control when performing a diagnostic digest is a simple but effective method for detecting plasmid multimers.

Aug 1, 2024 · Why do you get three bands when running uncut plasmid DNA on agarose gels? We'll explain why and how this can help you get successful DNA plasmid preps.

Download scientific diagram | The agarose gel shows that the monomer and dimer form of the plasmid have an absolutely identical restriction pattern, but the dimer migrates slower as can...

Aug 18, 2024 · You can identify the linear DNA form on an agarose gel by comparing uncut plasmid DNA with a sample of the plasmid that has been linearized using a restriction enzyme.

Completely digested plasmid DNA usually shows up a single band on the gel, a linear form of the plasmid, in its lane. Undigested plasmid may have two forms show up in its lane: a covalently closed circular dimer and a covalently closed circular monomer.

It looks like my histogram and/or virtual gel is reporting dimers, trimers, etc. Are these real concatemers/multimers, or just artifacts? How are the chromatograms (.ab1 files) useful? How do I interpret them? Why did my plasmid sample fail? What is your policy when plasmid samples fail?

Oct 15, 1999 · Plasmids may appear in different forms: circular with different degrees of coiling, partially cleaved or linear, and multimeric as concatamers or catenates. Capillary gel electrophoresis (CGE) of plasmid samples allows the determination of plasmid form distribution.

Apr 4, 2013 · A systematic comparison between an EGFP encoding plasmid DNA monomer and its dimer revealed two major findings: transfection of smaller monomeric plasmid DNA resulted in higher numbers of transfected cells compared to the dimer indicating an inverse effect of plasmid size on transfection efficiency.

May 1, 1981 · Two consecutive steps of agarose gel electrophoresis with a single DNA sample are used; use is made between the steps of ultraviolet light irradiation which introduces single-strand nicks in ethidium bromide-stained DNA, converting CCC into OC forms. Unambiguous assignments of OC and CCC forms are possible in samples containing several plasmids.

May 1, 2012 · I would expect the band at 10 kb to possibly be the relaxed form of the plasmid, with much of the plasmid DNA existing in a super-coiled conformation. If these are important samples, I would consider purifying them using cleaner methods, for example using a column.