Plasmid pJ2039 from Dr. Yolanda Schaerli's lab contains the insert NOT gate with sg-3 and is published in Nat Commun. 2020 Jun 2;11(1):2746. doi: 10.1038/s41467-020-16574-1. This plasmid is available through Addgene.

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The 2039 Series features high energy-handling capability, long and stable life performance and low capacitance of less than 1 pF. Bourns® GDTs are designed to prevent damage from transient disturbances by acting as a “crowbar” to create a short-to-ground circuit during conduction.

Jan 14, 2024 · Santos Moreno J, Tasiudi E, Stelling J, Schaerli Y. Multistable and dynamic CRISPRi-based synthetic circuits. Nat Commun. 2020;11:2746 pubmed publisher. pJ2039. Kanamycin. GenBank file with original annotations can be downloaded from the Resource Information section.

pj2039 (7459 bp) (from 1178-2354 bp) aaggccgcaaaagagattcttgaaatgcccggagaccactacattgggcatcgtttggtccgtaagacagaaggaaatattactgaacaggtcgaagacgctgtggc

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pj2039_n2only (7295 bp) (from 1285-2461 bp) cattgggtcaatatattcgttctcgcaagatgactgaaattgcccagaaattgaaagagtctaatgaacctattttgtacctggcggagcgttacggctttgaaagt

Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence. SnapGene File: Plasmid sequence and SnapGene enhanced annotations. Use with SnapGene software or the free Viewer to visualize additional data and align other sequences.

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