Incubate 20 min at 37°C with occasional mixing. While vortexing add 3 mL 0.1M Na-borate. Spin down nuclei. Aspirate supe and vortex pellet. While vortexing add 2 mL IFA/Tween 20. Spin …

The incorporation of BrdU is normally analyzed in flow cytometry by labelling with a conjugate anti-BrdU antibody and DNA dyes Propidium Iodide (PI) to perform cell cycle analysis.

Labeling and staining cells with BrdU, combined with total DNA staining by propidium iodide and analysis by flow cytometry 1 offers the most accurate measure of cells in the various stages of …

Jan 30, 2023 · BrdU (bromodeoxyuridine or 5-bromo-2'-deoxyuridine) is an analog of the nucleoside thymidine used in the BrdU assay to identify proliferating cells. BrdU labeling can …

Incorporated BrdU is further stained with fluoresceinated anti-BrdU antibodies and fluorescent DNA dye (e.g., propidium iodide, PI; 7-Aminoactinomycin D, 7-AAD) to separate the cells …

The BrdU Staining Kits for Flow Cytometry contains the necessary reagents and buffers for identifying and examining proliferating cells by flow cytometric analysis. Cycling cells are …

The main aim of this review is to guide the readers through the basic methods of the determination of cell cycle phases and estimation of their length, with a focus on the …

Incorporated BrdU can be detected using fluorescently labeled anti BrdU antibodies. When combined with a DNA stain such as PI or DAPI, the relative proportion of cells in S-phase can …

Bivariate cell cycle assay using cell cycle-specific antibodies and DNA dyes will not only reveals the distribution of cells at particular cycle phases, but also allows researchers to elucidate the …

The following protocol combines BrdU (FITC) staining of S phase cells in combination with propidium iodide for cell cycle analysis. This protocol is used courtesy of Prabhat Goswami, …