Phytochrome wild-type gene-[beta]-glucuronidase (PHY-GUS) gene fusions were used in transgenic Arabidopsis to compare the activity levels and light regulation of the PHYA and PHYB promoters and to identify the photoreceptors mediating this regulation.

Apr 16, 2014 · The expression patterns of PHYA:GUS showed interesting features, such as vascular bundle-specific and light-dependent expression as noted earlier, so in situ hybridization was performed to ensure the correspondence between the expression of PHYA:GUS and distribution of endogenous PHYA mRNA.

We determined the role of phyA and phyB in mediating negative photoregulation of the PHYA gene in Arabidopsis using phyA and phyB null mutants and have quantitatively defined the kinetics and fluence-rate dependence of FRc-light-imposed repression of this gene.

Mar 15, 2002 · In Arabidopsis, phytochrome A (phyA) is the primary photoreceptor mediating various plant responses to far‐red (FR) light. Here we show that phyA signaling involves a combinatorial action of downstream intermediates, which controls overlapping yet distinctive sets of FR responses.

To determine whether differential temporal or spatial expression patterns of the PHYA and PHYB genes contribute to this phenomenon the expression of PHYA-GUS and PHYB-GUS reporter genes has been examined in transgenic Arabidopsis.

Aug 8, 1999 · Here we describe a new Arabidopsis mutant, far1 (far -red-impaired response), which has reduced responsiveness to continuous far-red light, but responds normally to other light wavelengths. This phenotype implies a specific requirement for FAR1 in phyA signal transduction.

In this review we discuss the molecular mechanisms mediating PhyA-dependent signaling. Among the phytochrome receptors phyA displays unique properties.

Phytochrome wild-type gene-[beta]-glucuronidase (PHY-GUS) gene fusions were used in transgenic Arabidopsis to compare the activity levels and light regulation of the PHYA and PHYB promoters and to identify the photoreceptors mediating this regulation.

Their in situ hybridization assays corroborated GUS-detected expression pattern of PHYA, demonstrating that PHYA expression is down-regulated by light yet is still detectable in vascular bundles in light-grown seedlings.

The phytochrome genes PHYA and PHYB showed distinct patterns of tissue- and developmental stage-specific expression in rice. The PHYA promoter-GUS was expressed in all leaf tissues in etiolated seedlings, while its expression was restricted to vascular bundles in expanded leaves of light-grown seedlings.