Phage-immunoprecipitation sequencing (PhIP-Seq) technology is an innovative, high-throughput antibody detection method. It enables comprehensive analysis of individual antibody profiles. …

Sep 6, 2018 · Here, we provide a detailed protocol for performing ‘phage immunoprecipitation sequencing’ (PhIP-Seq), which is a powerful method for analyzing antibody-repertoire binding …

Phage immunoprecipitation sequencing (PhIP-Seq) is method that combines barcoded DNA high-throughput sequencing and proteomics to determine the levels of binding of antibodies to …

Jan 22, 2021 · While lacking in highly conformational, discontinuous, and post-translationally modified epitopes, PhIP-Seq enables high-resolution analysis of longer, higher quality …

May 11, 2022 · Phage ImmunoPrecipitation Sequencing (PhIP-Seq) is a high throughput serological technology that is revolutionizing the manner in which we track antibody profiles. In …

We present the phippery software suite for analyzing data from phage display methods that use immunoprecipitation and deep sequencing to capture antibody binding to peptides, often …

Sep 4, 2024 · Phage-immunoprecipitation sequencing (PhIP-Seq) technology is an innovative, high-throughput antibody detection method. It enables comprehensive analysis of individual …

Apr 10, 2024 · PhIP-seq is a novel powerful technique that uses well-defined proteomic libraries of equal length to probe circulating antibodies and pull-down interacting partners by …

We have used PhIP-Seq to identify novel self-antigens associated with autoimmune disease, to characterize the self-reactivity of broadly neutralizing HIV antibodies, and in a large …

Sep 15, 2022 · PhIP-Seq uses oligonucleotide library synthesis to encode proteome spanning peptide libraries for display on bacteriophages. These libraries are immunocaptured using an …