Jan 1, 2009 · Enhanced yellow fluorescent protein (EYFP) was generated by introducing the following four mutations in to GFP: T203Y, S65G, V68L, and S72A. Together these mutations alter the spectral properties and fluorescence intensity of EYFP as compared to GFP.

Topaz YFP, an enhanced yellow variant of GFP. Sequence Author: Thermo Fisher (Invitrogen) Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More Plasmid Sets. BsrGI is typically used at 37°C, but is even more active at 60°C. * Blocked by Dam methylation. * Blocked by Dcm methylation.

EYFP is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a very rapidly-maturing weak dimer with high acid sensitivity.

To aid with the discovery and analysis of plant PCD regulators, we have designed a novel cell death assay based on low cytosolic pH as a marker of PCD. The acidification that occurs in the cytosol during plant PCD was monitored by way of the extinction of YFP fluorescence at low pH.

Nov 1, 2024 · In this report we demonstrated that the 405-nm violet laser excitation could be used to discriminate GFP and YFP signals. Using the violet laser to distinguish GFP and YFP has several advantages. First, the standard setup of the flow cytometer could be used, obviating the need for exchanging filters and dichroic mirrors.

Yellow fluorescent protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. [1] Its excitation peak is 513 nm and its emission peak is 527 nm. [2]

Nov 7, 2022 · We solved the crystal structures of hfYFP (to 1.7-Å resolution), a monomeric variant, monomeric hyperfolder YFP (mhYFP) (1.6 Å), and a cysteine-free mGL variant called FOLD6 (1.2 Å), shedding...

Jan 1, 2002 · For YFP to be fully and stably fluorescent, mutations that decrease the sensitivity to both pH and Cl− are desired. Here we describe the development of an improved version of YFP named “Venus”.

Dec 24, 2010 · Human hepatitis B virus (HBV) induces a strong activation of Nrf2/ARE-regulated genes in vitro and in vivo. This is triggered by the HBV-regulatory proteins (HBx and LHBs) via c-Raf and MEK.

We first determined the maturation frequency of the monomeric citrine yellow fluorescent protein (YFP) variant in Arabidopsis to calibrate the frequency of two-step photobleaching events into a...