Jul 16, 2013 · Phosphoinositide-dependent kinase-1 (PDK1) is an essential master kinase recruited to the plasma membrane by the binding of its C-terminal PH domain to the signaling lipid phosphatidylinositol-3,4,5-trisphosphate (PIP3).

Apr 6, 2022 · We show that PDK1 trans -autophosphorylation is mediated by a PIP 3 -mediated face-to-face dimer. We report regulatory motifs in the kinase-PH interdomain linker that allosterically activate...

Phosphoinositide-Dependent Kinase-1 (PDK1) is an essential master kinase recruited to the plasma membrane by the binding of its C-terminal PH domain to the signaling lipid phosphatidylinositol-3,4-5-trisphosphate (PIP 3).

PDPK1 is a master kinase, which is crucial for the activation of AKT /PKB and many other AGC kinases including PKC, S6K, SGK. An important role for PDPK1 is in the signaling pathways activated by several growth factors and hormones including insulin signaling.

PDK1 interacts with phosphoinositides through a pleckstrin homology (PH) domain. To study the role of this interaction, we generated knock-in mice expressing a mutant of PDK1 incapable of binding phosphoinositides. The knock-in mice are significantly small, insulin resistant, and hyperinsulinemic.

Jun 6, 2023 · The PH domain of PDK1 is required for co-localization of PDK1 with one of its substrates, Akt, and for PDK1 dimerization (22, 45, 46, 48). Our present study showed that full-length PDK1 could adopt at least three conformations, which were determined by the position of the linker and the PH domain.

Aug 8, 2010 · 3-phosphoinositide-dependent protein kinase-1 (PDK1) is a central mediator of cellular signaling between phosphoinositide-3 kinase and various intracellular serine/threonine kinases, including protein kinase B, p70 ribosomal S6 kinase, serum and glucocorticoid-inducible kinase, and protein kinase C. PDK1 activates members of the AGC family of pr...

Oct 2, 2020 · PDK1 contains an N-terminal serine/threonine kinase domain followed by a PH domain. Following binding of the PH domain to PtdIns (3,4,5)P3 and PtdIns (3,4)P2, PDK1 activates these enzymes by phosphorylating a Ser/Thr residue in their activation loop.

Mar 22, 2022 · Here we report that ribosomal protein S6 kinase beta 1 (S6K1), a member of AGC kinases and downstream target of mechanistic target of rapamycin complex 1 (mTORC1), directly phosphorylates...

PDK1 is a 63 KDa Ser/Thr kinase with carboxyl terminal PH domain that binds with high affinity to PIP3-containing regions of plasma membrane and phosphorylates the colocalised AKT at its threonine 308 residue partially activating it (Mora et al., 2004).