Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site. For other reading frames, use pET-28a (+) or pET-28b (+). Sequence Author: MilliporeSigma …

There is a problem with the plasmid I received. What strain of bacteria does my stab contain? How can I be notified when a new material becomes available? What is virus associated DNA, and why do I have to order it? Welcome to Vector Database!

LOCUS pET-28c(+). 5367 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site.

Plasmid pET-28c S2 from Dr. Mingxu You's lab contains the insert RNA-based (p)ppGpp sensor and is published in Angew Chem Int Ed Engl. 2021 Nov 2;60 (45):24070-24074. doi: 10.1002/anie.202111170. Epub 2021 Oct 5. This plasmid is available through Addgene.

Plasmid pET28c-Ku from Dr. Harris Wang's lab contains the insert ku and is published in ACS Synth Biol. 2020 Sep 29. doi: 10.1021/acssynbio.0c00277. This plasmid is available through Addgene. Image: Illustrated plasmid map in PNG format

The pET-28a-c (+) vectors carry an N-terminal HIS TAG ® /thrombin/T7•Tag configuration plus an optional C-terminal HIS TAG sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB074).

The maps for pET-28b(+) and pET-28c(+) are the same as pET-28a(+) (shown) with the following exceptions: pET-28b(+) is a 5368bp plasmid; subtract 1bp from each site beyond BamH I at …

pET-28c (+) DNA - Novagen Novagen's pET-28a-c (+) vectors carry an N-terminal His•Tag®/thrombin/T7•Tag® configuration plus an optional C-terminal His•Tag sequence. - Find MSDS or SDS, a COA, data sheets and more information.

Browse pET-28c (+) DNA - Novagen and related products at MilliporeSigma.

Novagen′s pET-28a-c (+) vectors carry an N-terminal His•Tag /thrombin/T7•Tag configuration plus an optional C-terminal His•Tag sequence. Pricing and availability is not currently available. Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number.