
You have cloned a 1 kb gene into the pET17b vector - Chegg
You have cloned a 1 kb gene into the pET17b vector (see included map on the next page) using an HindIII site before the gene and an EcoRV site after the gene. You have confirmed that the …
Solved You have cloned a 1 kb gene into the pET17b vector - Chegg
You have cloned a 1 kb gene into the pET17b vector (see included map on the next page) using an HindIII site before the gene and an EcoRV site after the gene. You have confirmed that the …
You are designing PCR primers to amplify a gene using - Chegg
Question: You are designing PCR primers to amplify a gene using that gene cloned into the pET17b plasmid as a template. The coding strand of DNA seen below represents the gene …
ou are designing PCR primers to amplify a gene using - Chegg
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Solved Question 5 The DNA sequence of a gene that encodes a
Design suitable primers for the PCR: a) to clone the sequence coding for the native form of the protein between Ndel and Xhol; b) to fuse the protein to the His tag contained in PET17b, by …