Bacterial vector for inducible expression of N-terminally 10xHis-tagged proteins with a Factor Xa site. Sequence Author: MilliporeSigma (Novagen) Explore Over 2.7k Plasmids: pET & Duet …

Nterm Factor Xa cleavage site Bacterial vector for expressing 10xHis-tagged proteins. How was your experience today? Addgene is a nonprofit plasmid repository. We store and distribute high-quality plasmids from your colleagues.

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Apr 11, 2025 · Expression of the T7 polymerase is induced by the addition of the lactose analog IPTG to the bacterial culture. The pET vector exists as a low copy number plasmid in host E. coli, which reduces leaky expression before induction. The vector utilizes the T7lac promoter system for strong and tightly controlled gene expression.

General Plasmid Transform Protocol. 1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min. 2.

The pET-16b vector carries an N-terminal HIS TAG ® sequence followed by a Factor Xa site and three cloning sites. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB046). The pET Vectors are supplied as purified plasmid DNA (10 µg).

Plasmid pET16B.Pfu from Dr. Sung-Hou Kim's lab contains the insert Pfu DNA polymerase and is published in Acta Crystallogr D Biol Crystallogr. 1998 Sep 1. 54(Pt 5):986-8. This plasmid is available through Addgene.

Plasmid pET16b-PCNA from Dr. Andrew Deans's lab contains the insert PCNA and is published in PLoS One. 2020 Feb 24;15 (2):e0229000. doi: 10.1371/journal.pone.0229000. eCollection 2020. This plasmid is available through Addgene.

Aug 25, 2011 · The pET-16b vector includes an N-terminal His•Tag sequence, a Factor Xa site and three cloning sites. T7 expression region is reversed on circular map. You can help EcoliWiki by writing about the history of this plasmid. Novagen. See Help:References for how to manage references in EcoliWiki.

The pET-16b vector (Cat. No. 69662-3) carries an N-terminal His•Tag® sequence followed by a Factor Xa site and three cloning sites. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so …