Bacterial vector for inducible expression of N-terminally 10xHis-tagged proteins with a Factor Xa site. Sequence Author: MilliporeSigma (Novagen) Explore Over 2.7k Plasmids: pET & Duet Vectors (Novagen) | More Plasmid Sets. Sticky ends from different BsaI sites may not be compatible. BsaI can be used between 37°C and 50°C.

Nterm Factor Xa cleavage site Bacterial vector for expressing 10xHis-tagged proteins. How was your experience today? Addgene is a nonprofit plasmid repository.

5 days ago · The pET vector system is a powerful and widely used system for expressing recombinant proteins in E. coli. The gene of interest is cloned into the pET vector under the control of the strong bacteriophage T7 transcription and translation regulatory system.

Plasmid pET16B.Pfu from Dr. Sung-Hou Kim's lab contains the insert Pfu DNA polymerase and is published in Acta Crystallogr D Biol Crystallogr. 1998 Sep 1. 54 (Pt 5):986-8. This plasmid is available through Addgene.

Aug 25, 2011 · The pET-16b vector includes an N-terminal His•Tag sequence, a Factor Xa site and three cloning sites. T7 expression region is reversed on circular map. History

Provide pET-16b vector/plasmid map, full length sequence, antibiotic resistance, size and other information

coli dihydrodipicolinate reductase gene was successfully isolated and cloned into the histidine-tagged pET-16b expression vector. The 819 bp open-reading frame encoded the 273 amino acid polypeptide with subunit molecular mass of 28 kD.

Plasmid pET16b-PCNA from Dr. Andrew Deans's lab contains the insert PCNA and is published in PLoS One. 2020 Feb 24;15 (2):e0229000. doi: 10.1371/journal.pone.0229000. eCollection 2020. This plasmid is available through Addgene.

pET & Duet Vectors (Novagen) Bacterial expression and tagging vectors. Download Plasmid Set. Try SnapGene for Free. ... pET-16b. pET-30 Ek_LIC (linearized) pET-43.1 Ek_LIC (linearized) pET-9a. pET-17b. pET-30 Xa_LIC. pET-43.1a(+) pET-9b. pET-19b. pET-30 Xa_LIC (linearized) pET-43.1b(+) pET-9c. pET-20b(+)

The pET-16b vector (Cat. No. 69662-3) carries an N-terminal His•Tag® sequence followed by a Factor Xa site and three cloning sites. Unique sites are shown on the circle map.