Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site. For other reading frames, use pET-28b(+) or pET-28c(+).

pET Expression Systems provide core reagents needed for target gene cloning and expression. • pET vector DNA, 10 µg each of the indicated plasmids • Host bacterial strains BL21, BL21(DE3) and BL21(DE3)pLysS, glycerol stocks1, 2 • Induction control clone, glycerol stock

pET-28a(+) is a bacterial expression vector designed for the production of N-terminally 6xHis-tagged proteins with a thrombin cleavage site. This vector is particularly suitable for proteins that require post-translational processing to remove the His-tag.

The pET-28a-c(+) vectors carry an N-terminal His•Tag ®/thrombin/T7•Tag configuration plus an optional C-terminal His•Tag sequence. Unique sites are shown on the circle map.

Bacterial vector for inducible expression of N-terminally T7-tagged proteins. For other reading frames, use pET-21b(+) or pET-21c(+).

Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site. For other reading frames, use pET-28a(+) or pET-28c(+).

In the pET system, the protein coding sequence of interest is cloned downstream of the T7 promoter and gene 10 leader sequences, and then transformed into E. coli strains.

Basic Vector Information. An e.coli expression vector pET-28a fused with N-His, N-Thrombin, and N-sumo. Designed for Ni-NIA affinity purification and tag removal.

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Bacterial vector that encodes a signal sequence for inducible expression of proteins in the periplasm.