Detection of Phosphorylated ERK (Thr202/Tyr204) with HTRF reagents can be performed in a single plate used for culturing, stimulation and lysis. No washing steps are required. This HTS designed protocol enables miniaturization while maintaining robust HTRF quality.

The HTRF human IFN gamma kit is designed for the quantification of human IFN gamma release in cell supernatant.

HTRF®, is the premier TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) technology on the market. TR-FRET technologies such as HTRF bring together the sensitivity of fluorescence with the homogeneous nature of FRET (Fluorescence Resonance Energy Transfer) and the low background of time resolution.

HTRF (Homogeneous Time Resolved Fluorescence) is the most frequently used generic assay technology to measure analytes in a homogenous format, which is the ideal platform used for drug target studies in high-throughput screening (HTS).

Mar 13, 2025 · Explore how HTRF assays enhance high-throughput screening with precise biomolecular interaction analysis, optimized labeling strategies, and multiplexing capabilities.

developing a protein:protein interaction assay using HTRF® PPi reagents from Revvity. In an HTRF protein:protein interaction assay, one protein is labeled (directly or indirectly) with the donor, and the other protein is labeled (directly or indirectly) with the acceptor. When the two proteins interact, the

Jun 15, 2016 · The development of this newly described high-throughput homogeneous time-resolved fluorescence (HTRF) assay has allowed HTS to proceed and, from this, the identification and advancement of multiple validated series of tool compounds for PARG inhibition.

Apr 4, 2024 · In this chapter, we describe an HTRF protein-protein interaction (PPI) assay for the KRAS4b G-domain in the GppNHp-bound state and the RAF-1-RBD currently used for drug screens. Application of this assay contributes to the identification of lead compounds targeting the GTP-bound active state of K-RAS.

Homogeneous Time Resolved Fluorescence (HTRF) is a technique based on certain fluorescent dyes that exhibit unusually long fluorescence lifetimes. It measures reaction rates by eliminating background fluorescence interference and using non-radiative energy transfer or quenching between fluorescent rare earth metal chelates and different receptors.

Jan 23, 2025 · Homogeneous Time-Resolved Fluorescence (HTRF) is revolutionizing the way researchers approach complex biological assays. HTRF detection systems merge the principles of time-resolved fluorescence with the benefits of homogeneous assay formats, allowing for precise, reliable, and high-throughput data acquisition.