Apr 3, 2016 · The peak intensity is increasing proportionally with respect to the absorbance of the substance. Hence, by using the Beer-Lamberant law, we can induce there is a proportionality between peak intenstiy and the concentration. Is there any mathematical relation between the peak area and the concentration? Any help will be appreciated.

Jun 11, 2021 · Peak ratio calculation Discussions about chromatography data systems, LIMS, controllers, computer issues and related topics.

Nov 24, 2008 · Using peak area to estimate concentration Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

Jul 28, 2005 · The peak height has increased following the column cleaning and that was expected due to the higher plate count. Theoretically, the area should have remained constant i.e. the peak should have become correspondingly narrower.

Feb 2, 2007 · Peak area Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

Feb 16, 2010 · Peak Area confusion Discussions about GC and other "gas phase" separation techniques.

Apr 22, 2005 · In reverse order: % area is the percentage of the total peak area on the chromatogram made up by the peak for the analyte. e.g. if you have a peak of area 5000, another one of 4000 and your analyte peak is 1000 the % area for the analyte is 10%

Jan 31, 2021 · @Buttonwood, the area calculation by "half-peak width x peak height" gives really bad results in HPLC. He should rather use rectangular, Simpson's or trapezoidal method for Gaussian or exponentially modified Gaussians to get anywhere close to the true area.

Apr 25, 2020 · The peak height would be read from the chromatogram not calculated from the area. The relationship between height and area depends on the shape of the peak and would be very difficult to calculate.

Jan 17, 2008 · In addition, it depends on things that the area might depend on as well: flow rate, detector response, for example. Since there are less fundamental errors involved in peak area than peak height, I think the former is preferred.