The pat gene encodes the synthesis of phosphinothricin acetyltransferase (PAT) [32,47]. The herbicide glufosinate ammonium inhibits glutamine synthetase, which plays an important role in nitrogen metabolism in plants.

Phosphinothricin acetyltransferase gene (pat) is an important selectable marker and also a key herbicide trait gene in several commercial products. In maize, the transformation frequency (TF) using pat as a selectable marker is the lowest among the commonly used marker options including epsps, pmi o …

Phosphinothricin acetyltransferase (EC 2.3.1.183, PAT, PPT acetyltransferase, Pt-N-acetyltransferase, ac-Pt) is an enzyme with systematic name acetyl-CoA:phosphinothricin N-acetyltransferase. [1][2] This enzyme catalyses the following chemical reaction.

Gene: pat (syn) Gene Source: synthetic form of pat gene derived from Streptomyces viridochromogenes strain Tu 494 Product: phosphinothricin N-acetyltransferase (PAT) enzyme Function: eliminates herbicidal activity of glufosinate (phosphinothricin) herbicides by acetylation GM Trait: Glufosinate herbicide tolerance Events with gene pat (syn)

Oct 1, 1996 · The bar and pat genes, isolated from different Streptomyces species, both encode a phosphinothricin acetyltransferase (PAT) and are widely applied in plant genetic engineering.

Bialaphos resistance (BAR) and phosphinothricin acetyltransferase (PAT) genes, which convey resistance to the broad-spectrum herbicide phosphinothricin (also known as glufosinate) via N-acetylation, have been globally used in basic plant research ...

The bar and pat genes, isolated from different Streptomyces species, both encode a phosphinothricin acetyltransferase (PAT) and are widely applied in plant genetic engineering.

Sep 10, 2016 · The findings of this study suggest that the pat gene can be used as an efficient selectable marker when producing transgenic D. salina. Microalgae are widely used as bioreactors to produce heterologous proteins with high added value because they provide a simple mass culture system.

Oct 15, 1988 · The base composition of the pat gene is typical for Streptomyces [70.1 mol% (G + C) in total and 93.5 mol% (G + C) in the third position]. Translation of pat is initiated by a GTG codon which was identified by frameshift mutations in …

This document provides a comprehensive review of information and data relevant to the environmental risk assessment of the protein phosphinothricin-N-acetyl transferase (PAT) produced in genetically engineered (GE) plants by genes isolated from Streptomyces viridochromogenes (pat gene) or Streptomyces hygroscopicus (bar gene) and presents a ...