Plasmid pAS from Dr. Simon Elsaesser's lab contains the insert 4xPylT (Mma) cassette and is published in ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571. This plasmid is available through Addgene.

Plasmid pCas from Dr. Sheng Yang's lab contains the insert cas9 and is published in Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14. This plasmid is available through Addgene.

This paper will describe the development and application of a particular set of vectors which have been designed to achieve efficient expression of essentially any gene coding sequence in Escherichia coli.

Escherichia coli promoters that are more active at low temperature (15 to 20 degrees C) than at 37 degrees C were identified by using the transposon Tn5-lac to generate promoter fusions expressing beta-galactosidase (beta-Gal).

Efficient expression of heterologous genes in Escherichia coli. The pAS vector system and its applications

The present invention relates to PAS (Plasmid Acromobacter Secretion) factor variants, and more particularly to a PAS factor variant, a vector comprising the same and a method for mass...

One solution to this problem has been the development of recombinant vector systems which are designed to achieve efficient expression of cloned genes in a variety of biological systems.

Jun 13, 2016 · We designed a new type of polyadenylation-signal (PAS) trap vector system in living mice, the piggyBac (PB) (PAS-trapping (EGFP)) gene trapping vector, which takes advantage of the...

Aug 30, 2023 · Per-Arnt-Sim (PAS) domains are ubiquitous sensors found in diverse receptors in bacteria, archaea, and eukaryotes, but their origins, distribution across the tree of life, and extent of their functional diversity are not fully characterized.

The present invention relates to PAS (Plasmid Acromobacter Secretion) factor mutants, particularly to a PAS factor mutant, vectors carrying the same, and processes for preparing recombinant...