Aug 3, 2023 · 乳酸化修饰的抗体已经很成熟，有检测全部乳酸化修饰的（pan-Kla)，也有检测特异性位点乳酸化修饰的抗体，例如anti-H3K18la, anti-H3K27la,H4K12la等。 根据实验的精细程度，可以选择免疫斑点实验（左图）和WB实验（右图）。 相比其它检测方法，这个方法步骤相对简单，对样本制备的要求相对低，特别适合需要考虑剂量效应和时间效应的课题。 在免疫斑点 …

Recent findings by Zhang et al. state that lactate contributed to epigenetic regulation of genes by lactylating histone lysine residues and that lactate was found to be a precursor to histone lysine lactylation (Kla), which stimulated gene transcription from chromatin (Zhang et al., 2019); these findings demonstrate lactylation’s critical ...

Rabbit polyclonal antibody anti-Pan-Pan Lactic acid-Lysine is suitable for use in Western Blot and ELISA research applications.

Sep 11, 2023 · We aslo identified all histone Kla sites in senescent microglia, and found that both the H3K18 lactylation (H3K18la) and Pan-Kla were significantly up-regulated in senescent microglia and hippocampus tissues of naturally aged mice and AD modeling mice.

Oct 23, 2019 · Immunoblots using the pan anti-Kla antibody confirmed the presence of histone Kla and showed that histone Kla levels were increased in a dose-dependent fashion in response to exogenous...

Aug 1, 2024 · Kla emerges as a multifaceted regulator in tumor biology that influences transcription, non-histone protein functions, and metabolic processes within the TME. The 'dual engine' effect of Kla, involving both epigenetic and metabolic regulation, underscores its …

Oct 18, 2023 · The Pan-Kla levels in the CD4 + T cells of the EAU mice were up-regulated, and inhibiting glycolysis suppressed EAU progression. Thus, we supposed that the up-regulated Kla proteins played important roles in CD4 + T cell differentiation.

This study investigates whether lysine lactylation (Kla), a lactate-derived post-translational modification in macrophages, occurs in brain cells and if it does, whether Kla is induced by the stimuli that accompany changes in lactate levels.

May 17, 2024 · Histone lysine lactylation (Kla) is a newly discovered histone modification, which regulates gene expression in macrophages. In M1 macrophages, lactate is derived from incompletely oxidized glucose and then generate lactyl-CoA, which is transferred to lysine tails of histone proteins via the acetyl transferase p300.

Histone lysine lactylation (Kla) is a newly discovered histone modification, which regulates gene expression in macrophages. In M1 macrophages, lactate is derived from incompletely oxidized glucose and then generate lactyl-CoA, which is transferred to lysine tails of histone proteins via the acetyl transferase p300.