Examples of PAK1-regulated cellular processes include dynamic of actin and microtubule fibers, critical steps during cell cycle progression, motility and invasion, redox and energy metabolism, cell survival, angiogenesis, DNA-repair, hormone sensitivity, and gene expression.

The data presented here suggest that Paks (Pak1 and/or Pak2) critically contribute to this pathway by spatially and temporally organizing the interplay between actin, myosin, and FA dynamics to achieve an optimal balance between adhesion and contraction for maximal cell migration (Fig. 10 H).

Here we show that p21-activated kinase 1 (PAK1), a well-known actin regulator, promotes oligodendrocyte morphologic change and myelin production in the CNS. A combination of in vitro and in vivo models demonstrated that PAK1 is expressed throughout the oligodendrocyte lineage with highest expression in differentiated oligodendrocytes.

Here we report that even though Cdc42/Rac1 or Akt are not activated, phosphatidylinositol-3 (PI-3) kinase activation induces PAK1 kinase activity. Indeed, we demonstrate that PI-3 kinase associates with the N-terminal regulatory domain of PAK1 …

PAK1 is required for hepatocyte growth factor (HGF)-induced actin dynamics and proliferation of squamous NSCLC cells. EBC-1 cells were pretreated with Dox for 3 days prior to serum starvation for 16 hours and treatment with 50 ng/mL HGF for 1 hour.

Conclusions: These results indicate that Pak1, acting through a protein that contains an SH3 domain, regulates the structure of the actin cytoskeleton in mammalian cells, and may serve as an effector for Cdc42 and/or Rac1 in promoting cell motility.

Mar 3, 2013 · In this study, using new inhibitors against Cdc42 activation, PAK1 activation, and a new more selective inhibitor of MEK1/2, we derived the following pathway required for glucose-induced F-actin remodeling using both human and rodent islet β cells: Cdc42→PAK1→Raf-1-MEK1/2→ERK1/2.

Sep 9, 1997 · In this paper, we provide further evidence in support of a role for PAK1 in regulating actin assembly by demonstrating that endogenous PAK1 becomes co-localized with filamentous (F)-actin at the edge of lamellipodia and in dorsal and membrane ruffles induced by distinct upstream stimuli.

Nov 15, 2014 · These findings expand upon the existing model of actin remodeling in glucose uptake, by placing insulin-stimulated PAK1 signaling as a required upstream step to facilitate actin remodeling and subsequent cofilin dephosphorylation.

May 10, 2022 · Knockdown of PAK1 increased the live/dead cell ratio and promoted neurons survival. Our study showed that PAK1 is involved in ICH early secondary brain injury by affecting F-actin/G-actin ratio through the PAK1/LIMK1/cofilin pathway.