This study demonstrates that flow cytometric detection of PAIg combined with ROC curve analysis is a convenient, sensitive, and specific test, compared to previous methods, and it is useful for the differential diagnosis of thrombocytopenic patients.

An enzyme-linked immunoabsorbant assay (ELISA) for the detection of autoantibodies to platelet glycoprotein (GP) complexes was used in adults, 9 with immune-related thrombocytopenia and 16 with non-immune thrombocytopenia, in order to determine the true non-specific nature of the positive PAIg test.

Measurement of platelet-associated imunoglobulin (PAIg) has frequently been applied for the diagnosis of idiopathic thrombocytopenic purpura (ITP) and other immune thrombocytopenias. In the present study, a flow cytometry (FCM) analysis has been used to detect and characterize PAIg in 47 patients wi …

In order to improve the diagnostic efficiency of flow cytometric detection of PAIg, we used the reference value for ITP determined by ROC curve analysis from results of non-ITP patients and ITP patients. In this study, PAIgA was always detected with another immunoglobulin class in …

BACKGROUND AND OBJECTIVE: Flow cytometry (FC) to identify platelet-associated (PA) immunoglobulin (Ig) is a potentially useful diagnostic test for idiopathic thrombocytopenic purpura (ITP).

Jul 1, 2000 · Flow cytometry (FC) to identify platelet-associated (PA) immunoglobulin (Ig) is a potentially useful diagnostic test for idiopathic thrombocytopenic purpura (ITP).

Feb 1, 2009 · To explore the clinical utility of measuring platelet associated immunoglobulin (PAIG) at the time of diagnosis in children with immune thrombocytopenia (ITP). PAIG was measured by flow cytometry...

Since then, platelet-associated IgG (PAIgG) has been thought of as evidence of ITP. Although platelets from ITP patients show significantly higher PAIgG values than from normal control individuals, PAIgG is not specific for autoantibody because it …

We increased the specificity of flow cytometric detection of platelet-associated immunoglobulin (PAIg) by a combination of platelet gating and cutof for positivity determined by the use of receiver operating characteristic (ROC) curve analysis, and we evaluated the significance of elevated PAIg in non-immune thrombocytopenic purpura (ITP) patients.

Jun 25, 2015 · To explore the clinical utility of measuring platelet-associated immunoglobulin (PAIG) at the time of diagnosis in children with immune thrombocytopenia (ITP). PAIG was measured by flow cytometry using fluorescent murine anti-IgG and anti-IgM.