Potyvirus-Based Vectors for Heterologous Gene Expression in …
Dec 15, 2024 · The NIb / CP site consistently provided the highest expression efficiency for both GFP and Der p 5 across all tested host plants. Interestingly, the NIa / NIb site exhibited higher …
The Potato virus Y M(S)N(R) NIb-replicase is the elicitor of a veinal ...
May 1, 2002 · The PVY M(s)N(r) NIb-replicase protein was found to induce a hypersensitive response 10 days post inoculation in nematode resistant tobacco. None of the other PVX/PVY …
Generation of double-virus-resistant marker-free transgenic …
May 10, 2009 · In this study, we constructed a marker-free expression vector of a chimeric gene derived from the coat protein sequence of Potato virus X (PVX) and the nuclear inclusion …
The Potato virus Y MSNR NIb‐replicase is the elicitor of a veinal ...
Apr 30, 2002 · The PVY NIb N was inserted into the PVX expression vector and infectious transcripts were inoculated on to N. benthamiana. Both replicase constructs expressed from …
CRISPR-Cas12a Genome Editing at the Whole-Plant Level Using …
Oct 15, 2021 · These results indicate that a single PVX vector can supply the viral NIb activity that allows TEVΔNIb to replicate and systemically spread in wild-type N. benthamiana, as well as …
Full sequence of wild-type potato virus X (PVX; GenBank accession number MT799816) and its derived recombinant clones PVX::crFT, PVX::crXT1:crFT and PVX::NIb:crFT.
Stability of recombinant plant viruses containing genes of …
Apr 1, 2007 · The potato virus Y (PVY) NIb and HCPro open reading frames (ORFs) were introduced into PVX to generate PVX-NIb and PVX-HCPro, while the PVY NIb ORF was …
Simultaneous equimolar expression of multiple proteins in plants …
Oct 15, 2010 · Here, a new plant viral vector is described which overcomes these limitations. The technology is based on the replacement of the viral RNA polymerase (NIb) cistron of a …
CRISPR-Cas12a genome editing at the whole-plant level using two ...
Apr 19, 2021 · Here we describe successful DNA-free genome editing of Nicotiana benthamiana using two compatible RNA virus vectors, derived from tobacco etch virus (TEV; genus …
Engineering of a single PVX vector for complementation of …
Download scientific diagram | Engineering of a single PVX vector for complementation of defective TEVDNIb and expression of LbCas12a crRNA. (A) Schematic representation of …