Dec 6, 2022 · Phosphoribosyl pyrophosphate (PRPP) provides the indispensable ribose sugar for nucleotide metabolism. 10 Therefore, PRPS1/2-dependent generation of PRPP is important …

Feb 15, 2011 · We present a less time-consuming, more sensitive, and more precise method for the quantitative determination of nucleoside triphosphates (NTPs), 5-ribosyl-1-pyrophosphate …

Mar 27, 2023 · The hyperactive synthesis and use of nucleotide triphosphates (NTPs) and their deoxy counterparts (dNTPs) is a universal feature of cancer cells that is highly druggable.

Feb 12, 2025 · Ribose-5-phosphate from the pentose phosphate pathway (PPP) is converted into phosphoribosyl pyrophosphate (PRPP). The pathway then branches into purine and …

Nucleoside triphosphates that contain ribose as the sugar are conventionally abbreviated as NTPs, while nucleoside triphosphates containing deoxyribose as the sugar are abbreviated as …

Phosphoribosylpyrophosphate (PRPP), generated from the glucose-derived pentose phosphate pathway, is incorporated at the UMPS step in the cytosol. Mitochondrial TCA cycle …

Feb 17, 2017 · Describe the formation from ribonucleotides of deoxyribonucleotides (dNTPs). Indicate the regulatory role of PRPP in hepatic purine biosynthesis and the specific reaction of …

Nucle-oside diphosphate (NDP) kinases use ATP to phosphorylate dNDP to produce deoxynu-cleoside triphosphates (dNTPs). Purine nucleotides synthesis begins with 5-phosphoribosyl-1 …

Here, we report a novel LC-MS/MS quantitative method of each individual dNTP (dATP, dCTP, dGTP, and TTP) from cellular lysate, which circumvents many of these challenges.

Jun 14, 2024 · In this study, we investigate the substrate spectrum of a polyphosphate kinase for the conversion of non-canonical nucleotides. Guided by the structure, we created an enzyme …