Pyruvate kinase M2 activators promote tetramer formation and
Aug 26, 2012 · We show that expression of PKM1, the pyruvate kinase isoform with high constitutive activity, or exposure to published small-molecule PKM2 activators inhibits the …
Parkin Regulates the Activity of Pyruvate Kinase M2 - PMC
The PKM1 activity was analyzed with a pyruvate kinase activity assay kit. I, 293 cells were transfected with plasmid DNA expressing FLAG-PKM1 (F-PKM1), HA-Ub, and/or parkin. The …
PKM2 isoform-specific deletion reveals a differential requirement …
Muscle and 293T cell lysates serve as PKM1 and PKM2 protein controls, respectively. The size of full-length PKM-skip is marked with an arrowhead and PKM-skip degradation products are …
Acetylation Targets the M2 Isoform of Pyruvate Kinase for …
Flag-PKM2 was transfected into 293T cells followed by treatment with TSA and NAM for the indicated time, and PKM2 acetylation and protein levels were analyzed by western blot with …
Lactylation of PKM2 Suppresses Inflammatory Metabolic …
Previous studies showed that PKM1 was primarily found in the myocardium, and PKM2 was mainly expressed in 293T cells 45, 48. The data in Figure S2 confirms these findings. …
PKM2‐TMEM33 axis regulates lipid homeostasis in cancer cells …
Sep 6, 2021 · Herein, we discovered an ER transmembrane protein 33 (TMEM33) as a downstream effector of PKM2 that regulates activation of SREBPs and lipid metabolism. Loss …
PKM1 Confers Metabolic Advantages and Promotes Cell ... - Cell …
We show that PKM1, rather than PKM2, exhibits tumor-promoting function cell autonomously in a KrasG12D mouse model and in KRASG12V mouse embryo fibroblasts, and that pulmonary …
Flag-PKM2 was transfected into 293T cells followed by treatment with TSA and NAM for the indicated time, and PKM2 acetylation and protein levels were analyzed by western blot with …
Secreted pyruvate kinase M2 facilitates cell migration via PI3K…
Apr 3, 2015 · To investigate whether colon cancer cells secreted PKM1 or PKM2, the levels of PKM1 and PKM2 in conditioned medium were detected in DLD1, HT29, HCT116 cells and …
PRMT1 promotes Warburg effect by regulating the PKM2/PKM1
Jul 15, 2024 · PRMT1 serves as a glucose-inducible protein that promotes aerobic glycolysis via alternative splicing (AS) of PKM and NSCLC cell proliferation. Combination treatment with a …