Jan 4, 2024 · While it is well known to demethylate histones to activate transcription, PHF8 demethylates transcription factor YY1, functioning as a co-repressor for a large set of nuclear-coded ETC genes to drive mROS production and cancer development.

Depletion of PHF8 or YY1 dramatically decreased the cellular sensitivity to iPHF8 (Fig. 7F and SI Appendix, Fig. S8 S and T), which supports the specificity of iPHF8. iPHF8 treatment led to a significant increase of cellular H3K9me1/2 and H4K20me1 levels, but not H3K4me2, H3K36me3, and H3K79me2 levels (Fig. 7G and SI Appendix, Table S1).

Jan 2, 2024 · In the current study, we report that PHF8 can function as a transcriptional co- repressor to regulate a large subset of ETC genes by directly demethylating a non histone substrate YY1, which drives mROS production and cancer cell growth.

PHF8 requires its histone demethylase activity to enhance melanoma cell invasion. Transcriptomic and epigenomic analyses revealed that PHF8 orchestrates a molecular program that directly controls the TGFβ signaling pathway and, as a …

Jul 15, 2023 · Here, the authors suggest PHF8 as an epigenetic checkpoint and show that targeting PHF8 induces a viral mimicry response enhancing anti-tumour immunity in colorectal cancer.

Here, we report that, while it is well-known to demethylate histones to activate transcription, demethylase PHF8 also functions as a co-repressor for a large set of ETC genes via demethylating the transcription factor YY1 at a highly conserved lysine (K) residue, K258.

Jan 2, 2024 · Our data uncover a key epigenetic mechanism underlying ETC gene transcriptional regulation, demonstrating that targeting the PHF8/YY1 axis has great potential to treat cancers.

Jan 9, 2024 · Targeting the PHF8/YY1 axis suppresses cancer cell growth through modulation of ROS. The Nature Index tracks the affiliations of high-quality scientific articles. Updated monthly, the Nature...

Feb 18, 2022 · PHF8 is a histone demethylase that preferentially localizes at promoters and participates in cell cycle regulation by removing H4K20me1 from the promoters of E2F Transcription Factor 1 (E2F1)-regulated genes (12).

Jan 2, 2024 · In the current study, we report that PHF8 can function as a transcriptional co-repressor to regulate a large subset of ETC genes by directly demethylating a non histone substrate YY1, which drives mROS production and cancer cell growth.