Anti-PF4 and Serotonin Release Assay (SRA) for Diagnosing …
The anti-PF4 (ie, heparin-induced platelet antibody) test is an immunologic, enzyme-linked immunosorbent assay (ELISA) that detects IgG antibodies against the platelet factor 4 …
How I Diagnose and Manage HIT - American Society of …
Dec 10, 2011 · Three types of assays are highly sensitive for the diagnosis of HIT: the washed platelet activation assays (SRA and HIPA), the IgG-specific PF4-dependent EIAs (EIA-IgG), …
Serotonin-release assay-negative heparin-induced thrombocytopenia
Heparin-induced thrombocytopenia (HIT) is a prothrombotic drug reaction caused by platelet-activating anti-platelet factor 4 (PF4)/heparin antibodies. Pathogenic HIT antibodies can be …
Anti-PF4 ELISA-Negative, SRA-Positive Heparin-Induced …
Diagnosis is made with a screening antiplatelet factor (PF4) immunoassay and the serotonin-release assay (SRA) as a confirmatory test. Anti-PF4 assays have high sensitivity (98%) but …
Current Perspectives on Diagnostic Assays and Anti-PF4 …
Functional assays such as the 14 C serotonin–release assay (14 C-SRA) and heparin-induced platelet activation (HIPA) test are considered gold-standard tests for detecting pathological HIT …
Double-Negative (PF4-Negative/SRA-Negative) Heparin-Induced ...
Oct 14, 2022 · We describe a patient with a clinical course highly suspicious for HIT despite an initially weakly positive, then negative, PF4 antibody and repeatedly negative SRA. This …
Rethinking the Gold Standard: Real-World Performance of the …
Nov 15, 2022 · A new assay, the PF4-dependent P-selectin expression assay (PEA), has demonstrated comparable accuracy in HIT diagnosis employing a simpler laboratory …
How Common Is Serotonin Release Assay-Negative Heparin …
Heparin-induced thrombocytopenia (HIT), a prothrombotic drug reaction caused by platelet-activating anti-platelet factor 4 (PF4)/heparin antibodies, is commonly diagnosed with the …
False-Negative Platelet Factor 4 Antibodies and Serotonin Release Assay ...
Repeat PF4-H AB and SRA testing resulted positive. A positive SRA in the appropriate context is considered for the diagnosis of heparin-induced thrombocytopenia. This case report highlights …
The LabCorp Serotonin Release Assay (SRA) employs washed donor platelets and detects their activation by measuring the release of endogenous serotonin that is induced by addition of …
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