In this study, the expression of PDE4 subtypes in primary microglial cells was assayed. Primary microglial cells were then transfected with specific small interfering RNA (siRNA) against each PDE4 subtype. PDE4 subtype A-D knockdown was confirmed by …

PDE4 is the largest of the eleven mammalian PDE families. This review covers the properties and functions of the PDE4 family, highlighting procognitive and memory-enhancing effects associated with their inactivation.

In the present study, we rectify this by providing the crystal structures of the catalytic domains of PDE4A, PDE4B and PDE4D in complex with the PDE4 inhibitor NVP {4- [8- (3-nitrophenyl)- [1,7]naphthyridin-6-yl]benzoic acid} as well as the unliganded PDE4C structure.

Jan 5, 2011 · Phosphodiesterase-4 (PDE4) plays an important role in mediating memory via the control of intracellular cAMP signaling; inhibition of PDE4 enhances memory. However, development of PDE4 inhibitors as memory enhancers has been hampered by their major side effect of emesis.

Mar 23, 2024 · Meta-analysis of genome-wide association study data has implicated PDE4B in the pathogenesis of Alzheimer’s disease (AD), the leading cause of senile dementia. PDE4B encodes one of four subtypes of...

Oct 6, 2016 · In this study, the expression of PDE4 subtypes in primary microglial cells was assayed. Primary microglial cells were then transfected with specific small interfering RNA (siRNA) against each PDE4 subtype. PDE4 subtype A-D knockdown was confirmed by quantitative polymerase chain reaction.

Apr 15, 2007 · In this study, we show that anti-CD3/CD28 stimulation of human primary CD4 + T cells increases the expression of the PDE4 subtypes PDE4A, PDE4B, and PDE4D in a specific and time-dependent manner. PDE4A and PDE4D mRNAs as well as enzyme activities were up-regulated within 5 days, PDE4B showed a transient up-regulation with highest levels after 24 h.

Jan 24, 2013 · The purpose of this study was to determine the key roles of long-form PDE4Ds in antidepressant-like and cognition-enhancing effects in mice. The RNA interference (RNAi) technique was applied to knock-down long-form PDE4D given the lack of selective inhibitors of individual PDE4D variants.

Here, we examined whether long-form PDE4D knockdown by lentiviral RNA construct containing a specific microRNA/miRNA-mir hairpin structure (4DmiRNA) reversed memory impairment caused by amyloid-β1-42 (Aβ42) in mice using the Morris water maze (MWM) and novelty object recognition tests.

Nov 11, 2009 · To our surprise, 1 μM PDE4 inhibitor PDE4 siRNA or Smad3 inhibitor completely blocked TGF-β1–induced ROS production, thus establishing ROS as a target of PDE4 and Smad3 during EMT.