PDE4 proteins are distinguished from other PDEs by their high selectivity for cAMP over cGMP as substrate and their sensitivity to inhibition by the prototypal PDE4 inhibitor rolipram.

Apr 18, 2023 · Our findings revealed PDE4D might act as a critical mediator between NAFLD and associated kidney injury and indicated PDE4 inhibitor roflumilast as a potential therapeutic strategy for...

Jul 1, 2011 · PDE4 isoforms were immunopurified from lysates using anti-VSV agarose and immune complexes blotted for both the PDE4 proteins and LIS1–GFP. Specific co-capture of LIS1–GFP was observed in the immunoprecipitates (Fig. 1A).

Feb 11, 2014 · Here we identify the differential regulation of the PDE4D7 isoform during prostate cancer progression and uncover a role in controlling prostate cancer cell proliferation. PDE4 transcripts from...

In this review, we illustrate that PDE4 inhibitors retain their therapeutic potential in myriad diseases, but target identification should be more precise to establish selective inhibition of disease-affected PDE4 isoforms while avoiding isoforms involved in adverse effects.

Jul 1, 2022 · Taken together, α-mangostin derivative 4e, a PDE4 inhibitor, efficiently activated the cAMP/PKA pathway in neuronal cells, and promoted UPS activity as evidenced by enhanced degradation of UPS substrate Ub-G76V-GFP and Ub-R-GFP, as well as elevated proteasomal enzyme activity.

In this study, we found that if an N-terminal hydrophobic domain of ApPDE4 short-form is linked to the GFP-fused PH domain of OSBP, OSH1, and FAPP1, these proteins were localized to the plasma membrane as well as the TGN.

Results: Taken together, α-mangostin derivative 4e, a PDE4 inhibitor, efficiently activated the cAMP/PKA pathway in neuronal cells, and promoted UPS activity as evidenced by enhanced degradation of UPS substrate Ub-G76V-GFP and Ub-R-GFP, as well as elevated proteasomal enzyme activity.

In cells transfected to express wild-type PDE4A4 cAMP phosphodiesterase (PDE), the PDE4 selective inhibitor rolipram caused PDE4A4 to relocalise so as to form accretion foci. This process was followed in detail in living cells using a PDE4A4 …

Mar 24, 2024 · Phosphodiesterase 4 (PDE4), crucial in regulating the cyclic adenosine monophosphate (cAMP) signaling pathway, significantly impacts liver pathophysiology. This article highlights the comprehensive effects of PDE4 on liver health and disease, and its potential as a therapeutic agent.