Feb 14, 2018 · Probe and primer are two types of single-stranded oligonucleotides used in various types of PCR. Probes are used in the detection of specific DNA fragments in qPCR. Primers …

Among the most popular PCR probes out there are hydrolysis probes, Molecular Beacon probes, dual hybridization probes, Eclipse probes, Scorpion probes, and a few others. There are …

Oct 21, 2013 · Many factors can influence PCR experiments, including primer and probe characteristics such as location, length, interaction and self-folding, melting temperature, …

Learn about PCR primer and probe chemistries for real-time PCR. Discover DNA primers with fluorescent DNA-binding dyes, hydrolysis probes and molecular beacons.

Apr 7, 2015 · Learn what fluorophores are compatible with various real-time PCR instruments and best practices for picking reporter dyes and quenchers on qPCR probes.

A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It …

Review options for custom-synthesized oligos, primers, probes, and genes for PCR, cloning, hybridization, real-time PCR, protein expression. Also, access our easy oligo ordering portal or …

Nov 9, 2017 · PCR (Polymerase Chain Reaction) is a revolutionary method developed by Kary Mullis in the 1980s. PCR is based on using the ability of DNA polymerase to synthesize new …

Probe-based quantitative PCR (qPCR) uses real-time fluorescence from 5ʹ-3ʹ exonuclease cleavage of a fluorescently-labeled, target-specific probe to measure DNA amplification at …

PCR probes are DNA or RNA sequences labeled with a reporter molecule. The reporter is a molecule that emits fluorescence when hybridized to the target sequence. These probes are …