3 days ago · PARP Antibody detects endogenous levels of full length PARP1 (116 kDa), as well as the large fragment (89 kDa) of PARP1 resulting from caspase cleavage. The antibody does not cross-react with related proteins or other PARP isoforms.

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5).

Out of 17 members, PARP‐1 (113 kDa) was the first characterized and extensively studied enzyme recognized to play an essential role in DNA repair. 11 PARP‐1 and PARP‐2 share ~69% homology in the catalytic domain and they are documented as vital proteins in DNA repair system, 2 while PARP‐3 is reported to be a mono‐ADP‐ribosylating ...

Jun 23, 2023 · PARP-1 contains several conserved and functionally distinct domains, including a self-modifying domain, a 55-kDa catalytic domain, and two zinc finger domains that bind DNA (Kotova et al., 2010). PARP-1 is mobilized to the locations of DNA damage.

6 days ago · PARP (46D11) Rabbit mAb detects endogenous levels of total full-length PARP-1 and the large fragment (89 kDa) produced by caspase cleavage at Asp214. This antibody does not cross-react with PARP-2 and PARP-3.

Poly (ADP-ribose) polymerase-1 (PARP-1) and its cleavage products regulate cell viability and NF-kB activity when expressed in neurons. PARP-1 cleavage generates a 24kDa (PARP-1 24) and an 89kDa fragment (PARP-1 89).

2 days ago · Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb detects endogenous levels of the large fragment (89 kDa) of human PARP1 protein produced by caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoforms.

During caspase-dependent apoptosis, PARP1 is cleaved by caspases 3 and 7 at its caspase-cleavage site into 24-kDa and 89-kDa fragments (6–8). The 24-kDa PARP1 fragment contains the DNA-binding motif and the NLS, whereas the 89-kDa PARP1 fragment contains the automodification and catalytic domains.

PARP Antibody detects endogenous levels of full length PARP1 (116 kDa), as well as the large fragment (89 kDa) of PARP1 resulting from caspase cleavage. The antibody does not cross-react with related proteins or other PARP isoforms.