PA-GFP is a photoactivatable green fluorescent protein published in 2002, derived from Aequorea victoria.

Apr 25, 2017 · Since emission of green light is more phototoxic to cells, most scientists working on in vivo imaging models perfer the PA-RFPs over PA-GFP. Another class of irreversible PA-FPs are the photoconvertible fluorescent proteins (PC-FPs).

Sep 15, 2005 · This group of PAFPs consists of a photoactivatable variant of avGFP known as PA-GFP 8, a photoswitchable cyan fluorescent protein, (PS-CFP) 9, which was developed from a GFP-like protein from...

Here, we describe methods to tag and express proteins with PA-GFP, locally photoactivate the recombinant protein and record the dynamic behavior of the photoactivated molecules in live cells. Use of photoactivatable proteins is a powerful approach to examine dynamic processes, including spindle formation, in diverse cells.

May 4, 2017 · Phamret is a fusion protein, consisting of one PA-GFP coupled covalently to its FRET partner ECFP. Exposure to 458 nm wavelength leads to emission of ECFP at 480 nm. Adjacent photoactivation of PA-GFP is performed with a 405 nm laser beam.

Apr 1, 2009 · Crystal structures of the photoactivatable green fluorescent protein T203H variant (PA-GFP) have been solved in the native and photoactivated states, which under 488 nm illumination are dark and brightly fluorescent, respectively.

This report covers the two-photon activation and excitation properties of the PA-GFP, a photoactivatable variant of the Aequorea victoria green fluorescent protein in the spectral region from 720 to 920 nm.

Photoactivatable green fluorescent protein (paGFP) exhibits peculiar photo-physical properties making it an invaluable tool for protein/cell tracking in living cells/organisms. paGFP is normally excited in the violet range (405 nm), with an emission …

Oct 1, 2009 · The PA-GFP variant contains a Thr-to-His mutation that makes it devoid of green fluorescence until it is activated by violet light (390–415 nm).

Green fluorescent proteins (GFP) have a variety of applications in biological imaging and biosensing. All of the applications rely on the photophysical and photochemical properties of the GFP and its chromophore (Chro). Photoactivatable …