These data demonstrate that Ser15 phosphorylation is required for p53 function in the physiological context of p53-responsive promoters and suggest a key and possibly universal role even for low levels of this modification in promoting p53-transcription function.

Phosphorylation at Ser15 destabilizes the helix observed in p53 and leads to a less structured peptide (Figure 3C,D,F). p53 has a total helicity (α, 3 10) of 31%, whereas p53pSer15 is helical for only 4% of the simulation.

6 days ago · Phospho-p53 (Ser15) Antibody detects endogenous levels of p53 only when phosphorylated at serine 15. The antibody does not cross-react with p53 phosphorylated at other sites.

The intense phosphorylation of p53- Ser 15, combined with activation of ATM and Chk2 (involving centrioles) as well as phosphorylation of H2AX seen in the untreated mitotic cells, suggest mobilization of the DNA damage detection/repair machinery in controlling cytokinesis.

We propose that upon phosphorylation at Ser15 the p53 TAD1 peptide binds to CK1δ through an electrostatically driven induced fit mechanism resulting in a flanking fuzzy complex.

Dec 15, 1999 · These studies highlight phosphorylation of Ser15, a key phosphorylation target during the p53 activation process, as being critical for p53‐dependent transactivation.

The intense phosphorylation of p53-Ser15, combined with activation of ATM and Chk2 (involving centrioles) as well as phosphorylation of H2AX seen in the untreated mitotic cells, suggest mobilization of the DNA damage detection/repair machinery in controlling cytokinesis.

Dec 4, 1998 · The same N-terminal domain of p53 has recently been shown to be phosphorylated at Ser15 in response to gamma-irradiation. Remarkably, we now demonstrate that phosphorylation of p53 at Ser15 increases its ability to recruit CBP/p300.

We show that Ser15, but not Ser20, mutant p53 is unable to interact with B56γ and significantly reduces the ability of B56γ3 to inhibit cell proliferation and transformation, suggesting that Ser15 phosphorylation primes p53 for the p53-B56γ interaction and Thr55 dephosphorylation by PP2A.

Phosphorylation at Ser15 destabilizes the helix observed in p53 and leads to a less structured peptide (Figure 3C,D,F). p53 has a total heli-city (, 310) of 31%, whereas p53pSer15 is helical for only 4% of the