Jun 27, 2024 · Common essential genes, including DNMTs (for example, DNMT1), HDACs (for example, HDAC3), and bromodomain and extraterminal domain (BET) proteins (for example, BRD4), are among current epigenetic...

Here we report that the histone deacetylase SIRT1 regulates the activities of DNMT1, a key enzyme responsible for DNA methylation. In mass spectrometry analysis, 12 new acetylated lysine sites were identified in DNMT1. SIRT1 physically associates with DNMT1 and can deacetylate acetylated DNMT1 in vitro and in vivo.

Acetylated HA-DNMT1 was significantly increased in cells cotransfected with PCAF over that in cells cotransfected with p300 or vector alone (Fig. 1 A). Amounts of HA-DNMT1 were similar under all three conditions.

Mar 5, 2017 · Major finding: Oxidative stress targets TET2 and DNMT1 to chromatin to increase both DNA methylation and 5hmC. Mechanism: p300-mediated acetylation of TET2 at K110 enhances TET2 activity, stability, and binding to DNMT1. Impact: HDAC inhibitors may enhance TET2 activity to suppress abnormal DNA methylation in patients with cancer.

Dec 15, 2015 · Mechanistically, p300-histone acetyltransferase acetylates STAT3, which, in turn, upregulates and activates the DNMT3b DNA methyltransferase. DNMT3b methylates CpG sites of the SHP-1...

Our review manuscript provides a comprehensive understanding of key factors regulating DNMT isoforms in breast tumor pathology and documents unsolved issues.

We demonstrated that the DNMT inhibitor 5-Aza-CdR significantly facilitated RANKL-induced OCP cell differentiation through CpG demethylation and transcriptional activation of MMP-9 gene. Contrarily, however, treatment of OCP cells with the HDAC inhibitor TSA …

Nov 24, 2003 · Our results indicate that expression of the Dnmt 1 gene is controled by Sp1 and Sp3 and that p300 is involved in the activation by Sp3. histone acetyltransferase. Transcription is regulated by the combinational actions of proteins that …

Jan 5, 2017 · Ellagic acid (TBBD) (35) and pyrazole-containing derivatives have been elucidated as inhibitors of coactivator-associated arginine methyltransferase (CARM1, also known as PRMT4), responsible for catalyzing H3R17me2 and H3R26me2, modifying non-histone proteins (e.g., p300/CBP (CREB-binding protein) and SRC-3 (Steroid receptor coactivator-3)), co ...

The expression of Dnmt1 gene is observed at early S phase. Sp1 is recruited mainly at the G1 phase and Sp3 is recruited at the early S phase. p300 is also obviously recruited at the second S phase. These data indicated that the regulators of Dnmt1 gene were controlled in cell-cycle dependent manner.