Our results suggest a distinct mechanism of p21 degradation in keratinocytes by UVB, and this p21 degradation may significantly enhance UVB-induced apoptosis of premalignant keratinocytes with a p53 defect to eliminate damaged cells and therefore prevent skin cancer development.

Ablation of the endogenous p21 protein has been shown to give rise to higher efficacy of DNA damage repair, enhanced clonogenic survival, and reduced apoptosis in response to UVB irradiation in p53−/− DLD-1 cells (Therrien et al., 2001).

Jan 25, 2024 · The WB results of p16 and qPCR results of p21 showed that Dox alleviated the cycle arrest and aging of HaCaT cells caused by UVB. In summary, we can conclude that Dox has excellent performance in alleviating the photoaging of HaCaT cells induced by UVB.

Our results reveal that lincRNA-p21 is highly inducible by UVB through a p53-dependent mechanism and that lincRNA-p21 has a key role in triggering UVB-induced apoptosis in human and mouse keratinocytes.

Apr 30, 2015 · Results: We found that UVB exposure induced significant p21 downregulation (p<0.05) and was associated with significantly increased apoptosis, significantly decreased proliferation, and significantly increased G2 phase arrest (p<0.05) in UVB-irradiated HaCaT keratinocytes. p21 silencing significantly promoted apoptosis, significantly inhibited ...

To elucidate an effect of ultraviolet B (UVB) radiation on p16 expression and its relation to p21, and proliferating cell nuclear antigen (PCNA), immunohistochemical and western blot analyses were performed on UVB-irradiated normal human epidermis and …

Our findings indicate that, in contrast to UVC, UVB induces p21 degradation independent of the ATR/ATM pathway. It appears that the MDM2 and GSK3β pathways are involved in UVB-induced p21 degradation, and restoring p21 reduces UVB-induced apoptosis of keratinocytes.

Jan 23, 2008 · The p21 CDKN1A protein was rapidly degraded within 1 h after UVB irradiation of (−)IGF-1R keratinocytes. In contrast, the level of p21 CDKN1A in (+)IGF-1R keratinocytes after UVB irradiation steadily increased over the entire 48-h assay.

Mar 19, 2015 · Our results reveal that lincRNA-p21 is highly inducible by UVB through a p53-dependent mechanism and that lincRNA-p21 has a key role in triggering UVB-induced apoptosis in human and mouse keratinocytes.

UVB induces p21 degradation via a proteasomal pathway. To investigate whether the decreased p21 protein levels were caused by reduced protein stability, we measured the half-life of p21 in UVB-irradiated HaCaT cells.